Distribution of viral RNA in the spinal cord of DBA/2 mice developing biphasic paralysis following infection with the D variant of encephalomyocarditis virus (EMC-D)

M. Takeda, R. Miura, K. Shiota, K. Hirasawa, M. J. Lee, S. I. Itagaki, K. Doi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

DBA/2 mice infected with the D variant of encephalomyocarditis virus (EMC-D) (101PFU/head) developed biphasic hind limb paralysis. As a first step in clarifying its pathogenesis, we examined the distribution of viral RNA in the spinal cord using in situ hybridization. At 3 days post inoculation (DPI), in the spinal cord of mice showing slight paralysis, viral RNA was observed in capillary endothelial cells and a few adjacent glia cells in the funiculus lateralis from thoracic to lumbar enlargement. At 7 DPI, in the spinal cord of mice showing apparent paralysis, viral RNA was observed in a larger number of glia cells in the demyelinated lesion associated with infiltration of macrophages in the funiculus lateralis and in a small number of degenerated neurons in the cornu ventrale. In the funiculus lateralis, viral RNA could not be observed after 28 DPI. On the other hand, viral RNA was observed in degenerated neurons in the cornu ventrale of mice showing the second phase paralysis at 42 DPI. Many CD4+T cells infiltrated around these degenerated neurons. These results suggest that: (1) the viral entry zone was the capillary endothelial cells in the funiculus lateralis; (2) first phase paralysis was due to demyelination caused by EMC-D and associated with macrophage infiltration; (3) second phase paralysis was due to degeneration of motor neurons bearing viral RNA associated with infiltration by CD4+T cells.

Original languageEnglish
Pages (from-to)441-447
Number of pages7
JournalInternational Journal of Experimental Pathology
Volume76
Issue number6
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Encephalomyocarditis virus
Inbred DBA Mouse
Viral RNA
Paralysis
Spinal Cord
Infection
Neurons
Neuroglia
Endothelial Cells
Macrophages
T-Lymphocytes
Motor Neurons
Demyelinating Diseases
In Situ Hybridization
Thorax
Extremities
Cell Count
Head

Keywords

  • Biphasic paralysis
  • EMC-D
  • In situ hybridization
  • Viral RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Distribution of viral RNA in the spinal cord of DBA/2 mice developing biphasic paralysis following infection with the D variant of encephalomyocarditis virus (EMC-D). / Takeda, M.; Miura, R.; Shiota, K.; Hirasawa, K.; Lee, M. J.; Itagaki, S. I.; Doi, K.

In: International Journal of Experimental Pathology, Vol. 76, No. 6, 1995, p. 441-447.

Research output: Contribution to journalArticle

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