Abstract
Four subtypes of melatonin receptor genes (Mel1a 1.4, Mel1a 1.7, Mel1b, and Mel1c) are considered to be expressed to mediate various physiological functions of melatonin in goldfish (Carassius auratus). To examine their tissue distribution and diurnal changes in expression levels, we cloned partial gene fragments for these melatonin receptor subtypes, and established specific RT-PCR and quantitative real-time PCR systems. Mel1a 1.4 and Mel1b were predominantly expressed in various neuronal and peripheral tissues, while Mel1a 1.7 and Mel1c were expressed in the restricted tissues. All subtype genes were expressed in the optic tectum, diencephalon, mesencephalon, vagal lobe, retina and spleen. The real-time PCR analyses showed that significant differences among time were observed for Mel1a 1.4 in the optic tectum and for Mel1a 1.7 and Mel1b in the retina. In the retina, the levels of Mel1a 1.7 and Mel1b mRNAs showed diurnal changes with one peak at ZT24. The present results show differential distribution of four subtypes of melatonin receptor mRNAs in the neuronal and peripheral tissues. However, the expressions of all subtype genes in the retinorecipient brain regions and retina reinforce the role of the melatonin receptor in processing visual information. Furthermore, the present study demonstrates diurnal expressions of the major subtype genes, i.e. Mel1a 1.4 in the optic tectum and Mel1a 1.7 in the retina.
| Original language | English |
|---|---|
| Pages (from-to) | 219-224 |
| Number of pages | 6 |
| Journal | Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology |
| Volume | 152 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Feb |
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Keywords
- Brain
- Diurnal rhythm
- Goldfish
- Melatonin receptor
- Optic tectum
- Retina
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Physiology
Cite this
Diurnal expressions of four subtypes of melatonin receptor genes in the optic tectum and retina of goldfish. / Ikegami, Taro; Azuma, Kyoichi; Nakamura, Masahisa; Suzuki, Nobuo; Hattori, Atsuhiko; Ando, Hironori.
In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 152, No. 2, 02.2009, p. 219-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diurnal expressions of four subtypes of melatonin receptor genes in the optic tectum and retina of goldfish
AU - Ikegami, Taro
AU - Azuma, Kyoichi
AU - Nakamura, Masahisa
AU - Suzuki, Nobuo
AU - Hattori, Atsuhiko
AU - Ando, Hironori
PY - 2009/2
Y1 - 2009/2
N2 - Four subtypes of melatonin receptor genes (Mel1a 1.4, Mel1a 1.7, Mel1b, and Mel1c) are considered to be expressed to mediate various physiological functions of melatonin in goldfish (Carassius auratus). To examine their tissue distribution and diurnal changes in expression levels, we cloned partial gene fragments for these melatonin receptor subtypes, and established specific RT-PCR and quantitative real-time PCR systems. Mel1a 1.4 and Mel1b were predominantly expressed in various neuronal and peripheral tissues, while Mel1a 1.7 and Mel1c were expressed in the restricted tissues. All subtype genes were expressed in the optic tectum, diencephalon, mesencephalon, vagal lobe, retina and spleen. The real-time PCR analyses showed that significant differences among time were observed for Mel1a 1.4 in the optic tectum and for Mel1a 1.7 and Mel1b in the retina. In the retina, the levels of Mel1a 1.7 and Mel1b mRNAs showed diurnal changes with one peak at ZT24. The present results show differential distribution of four subtypes of melatonin receptor mRNAs in the neuronal and peripheral tissues. However, the expressions of all subtype genes in the retinorecipient brain regions and retina reinforce the role of the melatonin receptor in processing visual information. Furthermore, the present study demonstrates diurnal expressions of the major subtype genes, i.e. Mel1a 1.4 in the optic tectum and Mel1a 1.7 in the retina.
AB - Four subtypes of melatonin receptor genes (Mel1a 1.4, Mel1a 1.7, Mel1b, and Mel1c) are considered to be expressed to mediate various physiological functions of melatonin in goldfish (Carassius auratus). To examine their tissue distribution and diurnal changes in expression levels, we cloned partial gene fragments for these melatonin receptor subtypes, and established specific RT-PCR and quantitative real-time PCR systems. Mel1a 1.4 and Mel1b were predominantly expressed in various neuronal and peripheral tissues, while Mel1a 1.7 and Mel1c were expressed in the restricted tissues. All subtype genes were expressed in the optic tectum, diencephalon, mesencephalon, vagal lobe, retina and spleen. The real-time PCR analyses showed that significant differences among time were observed for Mel1a 1.4 in the optic tectum and for Mel1a 1.7 and Mel1b in the retina. In the retina, the levels of Mel1a 1.7 and Mel1b mRNAs showed diurnal changes with one peak at ZT24. The present results show differential distribution of four subtypes of melatonin receptor mRNAs in the neuronal and peripheral tissues. However, the expressions of all subtype genes in the retinorecipient brain regions and retina reinforce the role of the melatonin receptor in processing visual information. Furthermore, the present study demonstrates diurnal expressions of the major subtype genes, i.e. Mel1a 1.4 in the optic tectum and Mel1a 1.7 in the retina.
KW - Brain
KW - Diurnal rhythm
KW - Goldfish
KW - Melatonin receptor
KW - Optic tectum
KW - Retina
UR - http://www.scopus.com/inward/record.url?scp=57549097839&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57549097839&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2008.09.030
DO - 10.1016/j.cbpa.2008.09.030
M3 - Article
C2 - 18930834
AN - SCOPUS:57549097839
VL - 152
SP - 219
EP - 224
JO - Comparative Biochemistry and Physiology - A Physiology
JF - Comparative Biochemistry and Physiology - A Physiology
SN - 1095-6433
IS - 2
ER -