Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain

Toshiyuki Hamada; Koichiro Kako; Hisanori Wakamatsu; Shigenobu Shibata; Shigenori Watanabe; Norio Ishida

doi:10.1016/S0169-328X(98)00341-6

Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain

Toshiyuki Hamada, Koichiro Kako, Hisanori Wakamatsu, Shigenobu Shibata, Shigenori Watanabe, Norio Ishida^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

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Abstract

We previously reported the mammalian period repeat mRNA fluctuates during circadian time in the rat suprachiasmatic nucleus (SCN) which is considered to be a clock pacemaker in mammalian brain. Presently we discovered a period repeat sequence (PR) DNA-binding protein in the rat SCN nuclear extract. In the SCN, the binding activity of PR DNA-binding protein to (ACAGGC)₃ was most highest during the late day and most lowest during the late night by electro-mobility shift assay (EMSA). In the cortex nuclear extract, the binding of PR DNA-binding protein did not show a significant variation during a day. This is the first report to show the existence of diurnal regulated PR DNA-binding protein in the SCN.

Original language	English
Pages (from-to)	211-215
Number of pages	5
Journal	Molecular Brain Research
Volume	65
Issue number	2
DOIs	https://doi.org/10.1016/S0169-328X(98)00341-6
Publication status	Published - 1999 Mar 5

Keywords

Circadian rhythm
EMSA
Period repeat sequence
Suprachiasmatic nucleus

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/S0169-328X(98)00341-6

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title = "Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain",

abstract = "We previously reported the mammalian period repeat mRNA fluctuates during circadian time in the rat suprachiasmatic nucleus (SCN) which is considered to be a clock pacemaker in mammalian brain. Presently we discovered a period repeat sequence (PR) DNA-binding protein in the rat SCN nuclear extract. In the SCN, the binding activity of PR DNA-binding protein to (ACAGGC)3 was most highest during the late day and most lowest during the late night by electro-mobility shift assay (EMSA). In the cortex nuclear extract, the binding of PR DNA-binding protein did not show a significant variation during a day. This is the first report to show the existence of diurnal regulated PR DNA-binding protein in the SCN.",

keywords = "Circadian rhythm, EMSA, Period repeat sequence, Suprachiasmatic nucleus",

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T1 - Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain

AU - Hamada, Toshiyuki

AU - Kako, Koichiro

AU - Wakamatsu, Hisanori

AU - Shibata, Shigenobu

AU - Watanabe, Shigenori

AU - Ishida, Norio

PY - 1999/3/5

Y1 - 1999/3/5

N2 - We previously reported the mammalian period repeat mRNA fluctuates during circadian time in the rat suprachiasmatic nucleus (SCN) which is considered to be a clock pacemaker in mammalian brain. Presently we discovered a period repeat sequence (PR) DNA-binding protein in the rat SCN nuclear extract. In the SCN, the binding activity of PR DNA-binding protein to (ACAGGC)3 was most highest during the late day and most lowest during the late night by electro-mobility shift assay (EMSA). In the cortex nuclear extract, the binding of PR DNA-binding protein did not show a significant variation during a day. This is the first report to show the existence of diurnal regulated PR DNA-binding protein in the SCN.

AB - We previously reported the mammalian period repeat mRNA fluctuates during circadian time in the rat suprachiasmatic nucleus (SCN) which is considered to be a clock pacemaker in mammalian brain. Presently we discovered a period repeat sequence (PR) DNA-binding protein in the rat SCN nuclear extract. In the SCN, the binding activity of PR DNA-binding protein to (ACAGGC)3 was most highest during the late day and most lowest during the late night by electro-mobility shift assay (EMSA). In the cortex nuclear extract, the binding of PR DNA-binding protein did not show a significant variation during a day. This is the first report to show the existence of diurnal regulated PR DNA-binding protein in the SCN.

KW - Circadian rhythm

KW - EMSA

KW - Period repeat sequence

KW - Suprachiasmatic nucleus

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Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain

Abstract

Keywords

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