Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain

Toshiyuki Hamada, Koichiro Kako, Hisanori Wakamatsu, Shigenobu Shibata, Shigenori Watanabe, Norio Ishida

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We previously reported the mammalian period repeat mRNA fluctuates during circadian time in the rat suprachiasmatic nucleus (SCN) which is considered to be a clock pacemaker in mammalian brain. Presently we discovered a period repeat sequence (PR) DNA-binding protein in the rat SCN nuclear extract. In the SCN, the binding activity of PR DNA-binding protein to (ACAGGC)3 was most highest during the late day and most lowest during the late night by electro-mobility shift assay (EMSA). In the cortex nuclear extract, the binding of PR DNA-binding protein did not show a significant variation during a day. This is the first report to show the existence of diurnal regulated PR DNA-binding protein in the SCN.

Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalMolecular Brain Research
Volume65
Issue number2
DOIs
Publication statusPublished - 1999 Mar 5

Keywords

  • Circadian rhythm
  • EMSA
  • Period repeat sequence
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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