Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain

Toshiyuki Hamada, Koichiro Kako, Hisanori Wakamatsu, Shigenobu Shibata, Shigenori Watanabe, Norio Ishida

    Research output: Contribution to journalArticle

    Abstract

    We previously reported the mammalian period repeat mRNA fluctuates during circadian time in the rat suprachiasmatic nucleus (SCN) which is considered to be a clock pacemaker in mammalian brain. Presently we discovered a period repeat sequence (PR) DNA-binding protein in the rat SCN nuclear extract. In the SCN, the binding activity of PR DNA-binding protein to (ACAGGC)3 was most highest during the late day and most lowest during the late night by electro-mobility shift assay (EMSA). In the cortex nuclear extract, the binding of PR DNA-binding protein did not show a significant variation during a day. This is the first report to show the existence of diurnal regulated PR DNA-binding protein in the SCN.

    Original languageEnglish
    Pages (from-to)211-215
    Number of pages5
    JournalMolecular Brain Research
    Volume65
    Issue number2
    DOIs
    Publication statusPublished - 1999 Mar 5

    Fingerprint

    Suprachiasmatic Nucleus
    DNA-Binding Proteins
    Brain
    Electrophoretic Mobility Shift Assay
    Messenger RNA

    Keywords

    • Circadian rhythm
    • EMSA
    • Period repeat sequence
    • Suprachiasmatic nucleus

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience

    Cite this

    Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain. / Hamada, Toshiyuki; Kako, Koichiro; Wakamatsu, Hisanori; Shibata, Shigenobu; Watanabe, Shigenori; Ishida, Norio.

    In: Molecular Brain Research, Vol. 65, No. 2, 05.03.1999, p. 211-215.

    Research output: Contribution to journalArticle

    Hamada, Toshiyuki ; Kako, Koichiro ; Wakamatsu, Hisanori ; Shibata, Shigenobu ; Watanabe, Shigenori ; Ishida, Norio. / Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain. In: Molecular Brain Research. 1999 ; Vol. 65, No. 2. pp. 211-215.
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    AU - Ishida, Norio

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