Diurnal Variation in Neutrophil Function

Masahiko Shiraishi, Katsuhiko Suzuki, Tatsuya Abe, Takashi Kikuchi, Hideki Satoh, Shigeyuki Nakaji, Kazuo Sugawara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Neutrophil functions, including chemotaxis, reactive oxygen species (ROS)-producing capacity of neutrophils, and serum opsonic activity were investigated in 9 young healthy male volunteers. Venous blood of these volunteers was obtained under standardized conditions at 4-h intervals over a 24-h span. Neutrophil chemotaxis was evaluated by a modified Boyden technique, ROS-producing capacity of neutrophils and serum opsonic activity were measured by a simultaneous multiple measurement system based on luminol-dependent chemiluminescence and indicated by peak height and peak time. ROS-producing capacity of neutrophils and serum opsonic activity were activated in the daytime, and decreased from night to morning. There were negative correlations between the peak time of the luminol-dependent chemiluminescent response, neutrophil number (p<0.01) and segmented neutrophil number (p<0.01). On the other hand, no significant correlations were noted between serum opsonic activity and IgG, IgA, IgM, C3 or C4. In contrast, the peaks of neutrophil chemotaxis were at the wake-up time (6:00a.m.) and in the evening (6:00p.m.). This study indicates that diurnal variation of neutrophil function exists.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalEnvironmental Health and Preventive Medicine
Volume1
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Neutrophils
Oxygen
Chemiluminescence
Chemotaxis
Luminol
Reactive Oxygen Species
Blood
Serum
Luminescence
Immunoglobulin A
Immunoglobulin M
Volunteers
Healthy Volunteers
Immunoglobulin G

Keywords

  • Diurnal variation
  • Neutrophil chemotaxis
  • Reactive oxygen species-producing capacity of human neutrophils
  • Serum opsonic activity

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health

Cite this

Shiraishi, M., Suzuki, K., Abe, T., Kikuchi, T., Satoh, H., Nakaji, S., & Sugawara, K. (1996). Diurnal Variation in Neutrophil Function. Environmental Health and Preventive Medicine, 1(2), 65-70.

Diurnal Variation in Neutrophil Function. / Shiraishi, Masahiko; Suzuki, Katsuhiko; Abe, Tatsuya; Kikuchi, Takashi; Satoh, Hideki; Nakaji, Shigeyuki; Sugawara, Kazuo.

In: Environmental Health and Preventive Medicine, Vol. 1, No. 2, 1996, p. 65-70.

Research output: Contribution to journalArticle

Shiraishi, M, Suzuki, K, Abe, T, Kikuchi, T, Satoh, H, Nakaji, S & Sugawara, K 1996, 'Diurnal Variation in Neutrophil Function', Environmental Health and Preventive Medicine, vol. 1, no. 2, pp. 65-70.
Shiraishi M, Suzuki K, Abe T, Kikuchi T, Satoh H, Nakaji S et al. Diurnal Variation in Neutrophil Function. Environmental Health and Preventive Medicine. 1996;1(2):65-70.
Shiraishi, Masahiko ; Suzuki, Katsuhiko ; Abe, Tatsuya ; Kikuchi, Takashi ; Satoh, Hideki ; Nakaji, Shigeyuki ; Sugawara, Kazuo. / Diurnal Variation in Neutrophil Function. In: Environmental Health and Preventive Medicine. 1996 ; Vol. 1, No. 2. pp. 65-70.
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