DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

Y. Hirota; T. Ohyama

DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

Y. Hirota^*, T. Ohyama

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

Original language	English
Pages (from-to)	151-152
Number of pages	2
Journal	Nucleic acids symposium series
Issue number	27
Publication status	Published - 1992
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.",

abstract = "The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.",

author = "Y. Hirota and T. Ohyama",

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journal = "Nucleic acids symposium series",

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AU - Hirota, Y.

AU - Ohyama, T.

PY - 1992

Y1 - 1992

N2 - The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

AB - The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

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M3 - Article

C2 - 1289798

AN - SCOPUS:0027017632

SN - 0261-3166

SP - 151

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JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

IS - 27

ER -

DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

Abstract

ASJC Scopus subject areas

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