DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

Y. Hirota, T. Ohyama

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

Original languageEnglish
Pages (from-to)151-152
Number of pages2
JournalNucleic acids symposium series
Issue number27
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Medicine(all)

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