DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer

Brandon Yoza; Atsushi Arakaki; Tadashi Matsunaga

doi:10.1016/S0168-1656(02)00342-5

DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer

Brandon Yoza, Atsushi Arakaki, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

105 Citations (Scopus)

Abstract

A cascading hyperbranched polyamidoamine dendrimer was synthesized on the surface of bacterial magnetite from Magnetospirillum magneticum AMB-1 to allow enhanced extraction of DNA from fluid suspensions. Characterization of the synthesis revealed linear doubling of the surface amine charge from generations one through five starting with an amino silane initiator. Furthermore, transmission electron microscopy revealed clear dispersion of the single domain magnetite in aqueous solution. The dendrimer modified magnetic particles have been used to carry out magnetic separation of DNA. Binding and release efficiencies increased with the number of generations and those of bacterial magnetite modified with six generation dendrimer were 7 and 11 times respectively as many as those of bacterial magnetite modified with only amino silane.

Original language	English
Pages (from-to)	219-228
Number of pages	10
Journal	Journal of Biotechnology
Volume	101
Issue number	3
DOIs	https://doi.org/10.1016/S0168-1656(02)00342-5
Publication status	Published - 2003 Mar 20
Externally published	Yes

Keywords

Bacterial magnetite
Dendrimer
DNA extraction
Hyperbranched
Polyamidoamine

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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10.1016/S0168-1656(02)00342-5

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title = "DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer",

abstract = "A cascading hyperbranched polyamidoamine dendrimer was synthesized on the surface of bacterial magnetite from Magnetospirillum magneticum AMB-1 to allow enhanced extraction of DNA from fluid suspensions. Characterization of the synthesis revealed linear doubling of the surface amine charge from generations one through five starting with an amino silane initiator. Furthermore, transmission electron microscopy revealed clear dispersion of the single domain magnetite in aqueous solution. The dendrimer modified magnetic particles have been used to carry out magnetic separation of DNA. Binding and release efficiencies increased with the number of generations and those of bacterial magnetite modified with six generation dendrimer were 7 and 11 times respectively as many as those of bacterial magnetite modified with only amino silane.",

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AU - Yoza, Brandon

AU - Arakaki, Atsushi

AU - Matsunaga, Tadashi

PY - 2003/3/20

Y1 - 2003/3/20

N2 - A cascading hyperbranched polyamidoamine dendrimer was synthesized on the surface of bacterial magnetite from Magnetospirillum magneticum AMB-1 to allow enhanced extraction of DNA from fluid suspensions. Characterization of the synthesis revealed linear doubling of the surface amine charge from generations one through five starting with an amino silane initiator. Furthermore, transmission electron microscopy revealed clear dispersion of the single domain magnetite in aqueous solution. The dendrimer modified magnetic particles have been used to carry out magnetic separation of DNA. Binding and release efficiencies increased with the number of generations and those of bacterial magnetite modified with six generation dendrimer were 7 and 11 times respectively as many as those of bacterial magnetite modified with only amino silane.

AB - A cascading hyperbranched polyamidoamine dendrimer was synthesized on the surface of bacterial magnetite from Magnetospirillum magneticum AMB-1 to allow enhanced extraction of DNA from fluid suspensions. Characterization of the synthesis revealed linear doubling of the surface amine charge from generations one through five starting with an amino silane initiator. Furthermore, transmission electron microscopy revealed clear dispersion of the single domain magnetite in aqueous solution. The dendrimer modified magnetic particles have been used to carry out magnetic separation of DNA. Binding and release efficiencies increased with the number of generations and those of bacterial magnetite modified with six generation dendrimer were 7 and 11 times respectively as many as those of bacterial magnetite modified with only amino silane.

KW - Bacterial magnetite

KW - Dendrimer

KW - DNA extraction

KW - Hyperbranched

KW - Polyamidoamine

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DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer

Abstract

Keywords

ASJC Scopus subject areas

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