DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound

Brandon Yoza, Mitsufumi Matsumoto, Tadashi Matsunaga

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111 Citations (Scopus)

Abstract

Magnetic particles produced by magnetic bacteria have been used to carry out magnetic separation of DNA. Separation was achieved using magnetite coated with 3-aminopropyltriethoxysilane, N-(trimethoxy-silylpropyl) isothiouronium chloride or 3-[2-(2-aminoethyl)-ethylamino]-propyltrimethoxysilane (AEEA). The DNA binding efficiency increased with the number of amino groups present on the silane compounds and was 14 fold higher than with untreated magnetite. Addition of AEEA to aqueous solutions containing coated magnetite increased efficiency due to co-condensation of DNA. From 108 Escherichia coli cells, 7.1 μg of DNA was recovered using 100 μg of magnetite. E. coli DNA extracted with modified bacterial magnetite was suitable for restriction enzyme digestion and polymerase chain reaction (PCR). Ten replicate samples of E. coli cells were extracted using an automated magnetic robot and 9.5 μg of DNA was extracted using 100 μg of modified bacterial magnetite and possessed a 1.94 absorbance ratio (260:280 nm).

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalJournal of Biotechnology
Volume94
Issue number3
DOIs
Publication statusPublished - 2002 Apr 11
Externally publishedYes

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Keywords

  • Amino silane compound
  • Bacterial magnetite
  • DNA extraction

ASJC Scopus subject areas

  • Biotechnology

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