DNA hypomethylation circuit of the mouse oocyte-specific histone H1foo gene in female germ cell lineage

Chiaki Maeda, Shun Sato, Naoko Hattori, Satoshi Tanaka, Shintaro Yagi, Kunio Shiota

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The oocyte-specific subtype of the linker histone H1 is H1FOO, which constitutes a major part of oocyte chromatin. H1foo is expressed in growing oocytes, through fertilization, up until the two-cell embryo stage, when it is subsequently replaced by somatic H1 subtypes. To elucidate whether an epigenetic mechanism is involved in the limited expression of H1foo, we analyzed the dynamics of the DNA methylation status of the H1foo locus in germ and somatic cells. We identified a tissue-dependent and differentially methylated region (T-DMR) upstream of the H1foo gene, which was hypermethylated in sperm, somatic cells, and stem cell lines. This region was specifically unmethylated in the ovulated oocyte, where H1foo is expressed. 5-Aza-2′-deoxycytidine treatments and luciferase assays provided in vitro evidence that DNA methylation plays a role in repressing H1foo in nonexpressing cells. DNA methylation analyses of fetal germ cells revealed the T-DMR to be hypomethylated in female and male germ cells at Embryonic Day 9.5 (E9.5), whereas it was highly methylated in somatic cells at this stage. Intriguingly, the unmethylated status was continuously observed throughout oogenesis at E9.5, E12.5, E15.5, E18.5, in mature oocytes, and after fertilization, in E3.5 blastocysts. In comparison, male germ cells acquired methylation beyond E18.5. These data demonstrate a continuously unmethylated circuit at the H1foo locus in the female germline.

Original languageEnglish
Pages (from-to)816-821
Number of pages6
JournalBiology of Reproduction
Volume78
Issue number5
DOIs
Publication statusPublished - 2008 May
Externally publishedYes

Fingerprint

Cell Lineage
Germ Cells
Histones
Oocytes
DNA Methylation
DNA
Genes
decitabine
Fertilization
Oogenesis
Blastocyst
Luciferases
Epigenomics
Methylation
Chromatin
Spermatozoa
Stem Cells
Embryonic Structures
Cell Line

Keywords

  • DNA methylation
  • Epigenetics
  • Gametogenesis
  • Gene regulation
  • Histone H1
  • Oocyte
  • Oocyte development

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

DNA hypomethylation circuit of the mouse oocyte-specific histone H1foo gene in female germ cell lineage. / Maeda, Chiaki; Sato, Shun; Hattori, Naoko; Tanaka, Satoshi; Yagi, Shintaro; Shiota, Kunio.

In: Biology of Reproduction, Vol. 78, No. 5, 05.2008, p. 816-821.

Research output: Contribution to journalArticle

Maeda, Chiaki ; Sato, Shun ; Hattori, Naoko ; Tanaka, Satoshi ; Yagi, Shintaro ; Shiota, Kunio. / DNA hypomethylation circuit of the mouse oocyte-specific histone H1foo gene in female germ cell lineage. In: Biology of Reproduction. 2008 ; Vol. 78, No. 5. pp. 816-821.
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