Abstract
DNA methylation at CpG sequences is involved in tissue-specific and developmentally regulated gene expression. The Sry (sex-determining region on the Y chromosome) gene encodes a master protein for initiating testis differentiation in mammals, and its expression is restricted to gonadal somatic cells at 10.5-12.5 days postcoitum (dpe) in the mouse. We found that in vitro methylation of the 5′-flanking region of the Sry gene caused suppression of reporter activity, implying that Sry gene expression could be regulated by DNA methylation-mediated gene silencing. Bisulfite restriction mapping and sodium bisulfite sequencing revealed that the 5′-flanking region of the Sry gene was hypermethylated in the 8.5-dpc embryos in which the Sry gene was not expressed. Importantly, this region was specifically hypomethylated in the gonad at 11.5 dpc, while the hypermethylated status was maintained in tissues that do not express the Sry gene. We concluded that expression of the Sry gene is under the control of an epigenetic mechanism mediated by DNA methylation.
| Original language | English |
|---|---|
| Pages (from-to) | 22306-22313 |
| Number of pages | 8 |
| Journal | Journal of Biological Chemistry |
| Volume | 279 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - 2004 May 21 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
DNA methylation-mediated control of Sry gene expression in mouse gonadal development. / Nishino, Koichiro; Hattori, Naoko; Tanaka, Satoshi; Shiota, Kunio.
In: Journal of Biological Chemistry, Vol. 279, No. 21, 21.05.2004, p. 22306-22313.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA methylation-mediated control of Sry gene expression in mouse gonadal development
AU - Nishino, Koichiro
AU - Hattori, Naoko
AU - Tanaka, Satoshi
AU - Shiota, Kunio
PY - 2004/5/21
Y1 - 2004/5/21
N2 - DNA methylation at CpG sequences is involved in tissue-specific and developmentally regulated gene expression. The Sry (sex-determining region on the Y chromosome) gene encodes a master protein for initiating testis differentiation in mammals, and its expression is restricted to gonadal somatic cells at 10.5-12.5 days postcoitum (dpe) in the mouse. We found that in vitro methylation of the 5′-flanking region of the Sry gene caused suppression of reporter activity, implying that Sry gene expression could be regulated by DNA methylation-mediated gene silencing. Bisulfite restriction mapping and sodium bisulfite sequencing revealed that the 5′-flanking region of the Sry gene was hypermethylated in the 8.5-dpc embryos in which the Sry gene was not expressed. Importantly, this region was specifically hypomethylated in the gonad at 11.5 dpc, while the hypermethylated status was maintained in tissues that do not express the Sry gene. We concluded that expression of the Sry gene is under the control of an epigenetic mechanism mediated by DNA methylation.
AB - DNA methylation at CpG sequences is involved in tissue-specific and developmentally regulated gene expression. The Sry (sex-determining region on the Y chromosome) gene encodes a master protein for initiating testis differentiation in mammals, and its expression is restricted to gonadal somatic cells at 10.5-12.5 days postcoitum (dpe) in the mouse. We found that in vitro methylation of the 5′-flanking region of the Sry gene caused suppression of reporter activity, implying that Sry gene expression could be regulated by DNA methylation-mediated gene silencing. Bisulfite restriction mapping and sodium bisulfite sequencing revealed that the 5′-flanking region of the Sry gene was hypermethylated in the 8.5-dpc embryos in which the Sry gene was not expressed. Importantly, this region was specifically hypomethylated in the gonad at 11.5 dpc, while the hypermethylated status was maintained in tissues that do not express the Sry gene. We concluded that expression of the Sry gene is under the control of an epigenetic mechanism mediated by DNA methylation.
UR - http://www.scopus.com/inward/record.url?scp=2542450772&partnerID=8YFLogxK
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U2 - 10.1074/jbc.M309513200
DO - 10.1074/jbc.M309513200
M3 - Article
VL - 279
SP - 22306
EP - 22313
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 21
ER -