DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression

Shintaro Yagi, Keiji Hirabayashi, Shinya Sato, Wei Li, Yoko Takahashi, Tsutomu Hirakawa, Guoying Wu, Naoko Hattori, Naka Hattori, Jun Ohgane, Satoshi Tanaka, X. Shirley Liu, Kunio Shiota

Research output: Contribution to journalArticle

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Abstract

DNA methylation constitutes an important epigenetic regulation mechanism in many eukaryotes, although the extent of DNA methylation in the regulation of gene expression in the mammalian genome is poorly understood. We developed D-REAM, a genome-wide DNA methylation analysis method for tissue-dependent and differentially methylated region (T-DMR) profiling with restriction tag-mediated amplification in mouse tissues and cells. Using a mouse promoter tiling array covering a region from ?6 to 2.5 kb (∼30,000 transcription start sites), we found that over 3000 T-DMRs are hypomethylated in liver compared to cerebrum. The DNA methylation profile of liver was distinct from that of kidney and spleen. This hypomethylation profile marked genes that are specifically expressed in liver, including key transcription factors such as Hnf1a and Hnf4a. Genes with T-DMRs, especially those lacking CpG islands and those with HNF-1A binding motifis in their promoters, showed good correlation between their tissue-specific expression and liver hypomethylation status. T-DMRs located downstream from their transcription start sites also showed tissue-specific gene expression. These data indicate that multi-layered regulation of tissue-specific gene function could be elucidated by DNA methylation tissue profiling.

Original languageEnglish
Pages (from-to)1969-1978
Number of pages10
JournalGenome Research
Volume18
Issue number12
DOIs
Publication statusPublished - 2008 Dec
Externally publishedYes

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DNA Methylation
Genetic Promoter Regions
Gene Expression
Transcription Initiation Site
Liver
Genome
Genes
CpG Islands
Cerebrum
Gene Expression Regulation
Eukaryota
Epigenomics
Transcription Factors
Spleen
Kidney

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression. / Yagi, Shintaro; Hirabayashi, Keiji; Sato, Shinya; Li, Wei; Takahashi, Yoko; Hirakawa, Tsutomu; Wu, Guoying; Hattori, Naoko; Hattori, Naka; Ohgane, Jun; Tanaka, Satoshi; Liu, X. Shirley; Shiota, Kunio.

In: Genome Research, Vol. 18, No. 12, 12.2008, p. 1969-1978.

Research output: Contribution to journalArticle

Yagi, S, Hirabayashi, K, Sato, S, Li, W, Takahashi, Y, Hirakawa, T, Wu, G, Hattori, N, Hattori, N, Ohgane, J, Tanaka, S, Liu, XS & Shiota, K 2008, 'DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression', Genome Research, vol. 18, no. 12, pp. 1969-1978. https://doi.org/10.1101/gr.074070.107
Yagi, Shintaro ; Hirabayashi, Keiji ; Sato, Shinya ; Li, Wei ; Takahashi, Yoko ; Hirakawa, Tsutomu ; Wu, Guoying ; Hattori, Naoko ; Hattori, Naka ; Ohgane, Jun ; Tanaka, Satoshi ; Liu, X. Shirley ; Shiota, Kunio. / DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression. In: Genome Research. 2008 ; Vol. 18, No. 12. pp. 1969-1978.
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