DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803

Yukako Hihara; Kintake Sonoike; Minoru Kanehisa; Masahiko Ikeuchi

doi:10.1128/JB.185.5.1719-1725.2003

DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803

Yukako Hihara^*, Kintake Sonoike, Minoru Kanehisa, Masahiko Ikeuchi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

133 Citations (Scopus)

Abstract

Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

Original language	English
Pages (from-to)	1719-1725
Number of pages	7
Journal	Journal of Bacteriology
Volume	185
Issue number	5
DOIs	https://doi.org/10.1128/JB.185.5.1719-1725.2003
Publication status	Published - 2003 Mar
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/JB.185.5.1719-1725.2003

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title = "DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803",

abstract = "Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.",

author = "Yukako Hihara and Kintake Sonoike and Minoru Kanehisa and Masahiko Ikeuchi",

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AU - Sonoike, Kintake

AU - Kanehisa, Minoru

AU - Ikeuchi, Masahiko

PY - 2003/3

Y1 - 2003/3

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AB - Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

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DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803

Abstract

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