DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803

Yukako Hihara, Kintake Sonoike, Minoru Kanehisa, Masahiko Ikeuchi

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Abstract

Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

Original languageEnglish
Pages (from-to)1719-1725
Number of pages7
JournalJournal of Bacteriology
Volume185
Issue number5
DOIs
Publication statusPublished - 2003 Mar 1

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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