DNA microarray mediated transcriptional profiling of Nitrosomonas europaea in response to linear alkylbenzene sulfonates

Hidetoshi Urakawa, Junpei Matsumoto, Kazuho Inaba, Satoshi Tsuneda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Linear alkylbenzene sulfonates (LAS) constitute, quantitatively, the most important group of synthetic surfactants used today. We studied the gene expression of Nitrosomonas europaea in response to LAS using a DNA microarray because ammonia-oxidizers are thought to be more sensitive to LAS than other microorganisms. Our objective was to elucidate which genes are expressed for N. europaea in response to LAS exposure. Microarray analysis and real-time PCR assay revealed that c. 30 genes were significantly expressed after LAS exposure, in particular genes associated with energy production and conversion. Our findings demonstrate that physical disruption of membrane structures, which contain enzymes associated with energy production and conversion, might be an important explanation for the high sensitivity of N. europaea to LAS exposure.

Original languageEnglish
Pages (from-to)166-173
Number of pages8
JournalFEMS Microbiology Letters
Volume282
Issue number2
DOIs
Publication statusPublished - 2008 May 1

Keywords

  • Ammonia-oxidizing bacteria (AOB)
  • Anionic detergent
  • DNA microarrays
  • Linear alkylbenzene sulfonates (LAS)
  • Nitrosomonas europaea
  • Surfactants

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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