DNA sequence of the carA gene and the control region of carAB: Tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12

J. Piette, Hiroshi Nyunoya, C. J. Lusty, R. Cunin, G. Weyens, M. Crabeel, D. Charlier, N. Glansdorff, A. Piérard

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Abstract

The carAB operon of Escherichia coli K-12, which encodes the two subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrimidines. We describe the structure of the control region of carAB and the sequence of the carA gene. Nuclease S1 mapping experiments show that two adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstream promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.

Original languageEnglish
Pages (from-to)4134-4138
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number13 I
Publication statusPublished - 1984
Externally publishedYes

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Carbamyl Phosphate
Pyrimidines
Ligases
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Arginine
Escherichia coli
Glutamate-Ammonia Ligase
Operon
Codon
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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title = "DNA sequence of the carA gene and the control region of carAB: Tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12",
abstract = "The carAB operon of Escherichia coli K-12, which encodes the two subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrimidines. We describe the structure of the control region of carAB and the sequence of the carA gene. Nuclease S1 mapping experiments show that two adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstream promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.",
author = "J. Piette and Hiroshi Nyunoya and Lusty, {C. J.} and R. Cunin and G. Weyens and M. Crabeel and D. Charlier and N. Glansdorff and A. Pi{\'e}rard",
year = "1984",
language = "English",
volume = "81",
pages = "4134--4138",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
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number = "13 I",

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TY - JOUR

T1 - DNA sequence of the carA gene and the control region of carAB

T2 - Tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12

AU - Piette, J.

AU - Nyunoya, Hiroshi

AU - Lusty, C. J.

AU - Cunin, R.

AU - Weyens, G.

AU - Crabeel, M.

AU - Charlier, D.

AU - Glansdorff, N.

AU - Piérard, A.

PY - 1984

Y1 - 1984

N2 - The carAB operon of Escherichia coli K-12, which encodes the two subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrimidines. We describe the structure of the control region of carAB and the sequence of the carA gene. Nuclease S1 mapping experiments show that two adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstream promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.

AB - The carAB operon of Escherichia coli K-12, which encodes the two subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrimidines. We describe the structure of the control region of carAB and the sequence of the carA gene. Nuclease S1 mapping experiments show that two adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstream promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.

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