DNA sequence of the carA gene and the control region of carAB: Tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12

J. Piette, H. Nyunoya, C. J. Lusty, R. Cunin, G. Weyens, M. Crabeel, D. Charlier, N. Glansdorff, A. Piérard

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The carAB operon of Escherichia coli K-12, which encodes the two subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrimidines. We describe the structure of the control region of carAB and the sequence of the carA gene. Nuclease S1 mapping experiments show that two adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstream promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.

Original languageEnglish
Pages (from-to)4134-4138
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number13 I
DOIs
Publication statusPublished - 1984 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'DNA sequence of the carA gene and the control region of carAB: Tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12'. Together they form a unique fingerprint.

  • Cite this