Does calorie restriction induce mitochondrial biogenesis? A reevaluation

Chad R. Hancock, Dong Ho Han, Kazuhiko Higashida, Sang Hyun Kim, John O. Holloszy

Research output: Contribution to journalArticle

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Abstract

It has been reported that 30% calorie restriction (CR) for 3 mo results in large increases in mitochondrial biogenesis in heart, brain, liver, and adipose tissue, with concomitant increases in respiration and ATP synthesis. We found these results surprising, and performed this study to determine whether 30% CR does induce an increase in mitochondria in heart, brain, liver, adipose tissue, and/or skeletal muscle. To this end, we measured the levels of a range of mitochondrial proteins, and mRNAs. With the exception of long-chain acyl-CoA dehydrogenase protein level, which was increased ∼60% in adipose tissue, none of the mitochondrial proteins or mRNAs that we measured were increased in rats subjected to 30% CR for 14 wk. There was also no increase in citrate synthase activity. Because it is not possible to have an increase in mitochondria without any increase in key mitochondrial proteins, we conclude that 30% CR does not induce an increase in mitochondria in heart, brain, liver, adipose tissue, or skeletal muscle in laboratory rodents.

Original languageEnglish
Pages (from-to)785-791
Number of pages7
JournalFASEB Journal
Volume25
Issue number2
DOIs
Publication statusPublished - 2011 Feb

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Organelle Biogenesis
Mitochondria
Adipose Tissue
Mitochondrial Proteins
Liver
Tissue
Heart Mitochondria
Brain
Muscle
Skeletal Muscle
Long-Chain Acyl-CoA Dehydrogenase
Citrate (si)-Synthase
Rats
Rodentia
Respiration
Adenosine Triphosphate
Proteins
mitochondrial messenger RNA

Keywords

  • Aging
  • Citrate synthase
  • Cytochrome c
  • PGC-1α

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Hancock, C. R., Han, D. H., Higashida, K., Kim, S. H., & Holloszy, J. O. (2011). Does calorie restriction induce mitochondrial biogenesis? A reevaluation. FASEB Journal, 25(2), 785-791. https://doi.org/10.1096/fj.10-170415

Does calorie restriction induce mitochondrial biogenesis? A reevaluation. / Hancock, Chad R.; Han, Dong Ho; Higashida, Kazuhiko; Kim, Sang Hyun; Holloszy, John O.

In: FASEB Journal, Vol. 25, No. 2, 02.2011, p. 785-791.

Research output: Contribution to journalArticle

Hancock, CR, Han, DH, Higashida, K, Kim, SH & Holloszy, JO 2011, 'Does calorie restriction induce mitochondrial biogenesis? A reevaluation', FASEB Journal, vol. 25, no. 2, pp. 785-791. https://doi.org/10.1096/fj.10-170415
Hancock CR, Han DH, Higashida K, Kim SH, Holloszy JO. Does calorie restriction induce mitochondrial biogenesis? A reevaluation. FASEB Journal. 2011 Feb;25(2):785-791. https://doi.org/10.1096/fj.10-170415
Hancock, Chad R. ; Han, Dong Ho ; Higashida, Kazuhiko ; Kim, Sang Hyun ; Holloszy, John O. / Does calorie restriction induce mitochondrial biogenesis? A reevaluation. In: FASEB Journal. 2011 ; Vol. 25, No. 2. pp. 785-791.
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