Down-regulation of AMP-activated protein kinase by calorie restriction in rat liver

Kazuo To, Haruyoshi Yamaza, Toshimitsu Komatsu, Takahiro Hayashida, Hiroko Hayashi, Hiroaki Toyama, Takuya Chiba, Yoshikazu Higami, Isao Shimokawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

AMP-activated protein kinase (AMPK) may act as a key enzyme for metabolic adaptation to calorie restriction (CR) or reduced growth hormone (GH)-insulin-like growth factor (IGF)-1 signaling, an experimental intervention for lifespan extension in animals. We investigated the protein levels of AMPKα and a downstream enzyme, acetyl-CoA carboxylase (ACC), by immunoblotting of liver and quadriceps femoris muscle (QFM) extracts from 6-month-old wild-type (W) and GH-suppressed transgenic (Tg) Wistar rats fed ad libitum (AL) or 30% CR diets from 6 weeks of age. A modified alternate-day feeding regimen for CR yielded a fed-fasted cycle in CR rats, and therefore the effects of overnight fasting in W-AL rats were also evaluated. CR decreased threonine-172-phosphorylated AMPKα (p-AMPKα; an activated form) levels in the liver, whereas the CR-fed-fasted cycle or overnight fasting did not significantly affect the p-AMPKα level. In the QFM, the p-AMPKα level was slightly elevated in the CR-fasted phase, but greatly increased in the AL-fasted phase. Suppression of GH did not affect the p-AMPKα level. The phosphorylated-ACC levels did not alter in parallel with the p-AMPKα level, particularly in the liver. The present results suggest that CR down-regulates the AMPK activity in the liver on a long-term basis.

Original languageEnglish
Pages (from-to)1063-1071
Number of pages9
JournalExperimental Gerontology
Volume42
Issue number11
DOIs
Publication statusPublished - 2007 Nov
Externally publishedYes

Fingerprint

AMP-Activated Protein Kinases
Liver
Rats
Down-Regulation
Quadriceps Muscle
Growth Hormone
Acetyl-CoA Carboxylase
Fasting
Muscle
Transgenic Rats
Somatomedins
Enzymes
Threonine
Immunoblotting
Nutrition
Wistar Rats
Animals
Diet

Keywords

  • Acetyl-CoA carboxylase
  • Aging
  • AMP-activated protein kinase
  • Calorie restriction
  • Growth hormone
  • IGF-1
  • Metabolic adaptation

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

To, K., Yamaza, H., Komatsu, T., Hayashida, T., Hayashi, H., Toyama, H., ... Shimokawa, I. (2007). Down-regulation of AMP-activated protein kinase by calorie restriction in rat liver. Experimental Gerontology, 42(11), 1063-1071. https://doi.org/10.1016/j.exger.2007.07.003

Down-regulation of AMP-activated protein kinase by calorie restriction in rat liver. / To, Kazuo; Yamaza, Haruyoshi; Komatsu, Toshimitsu; Hayashida, Takahiro; Hayashi, Hiroko; Toyama, Hiroaki; Chiba, Takuya; Higami, Yoshikazu; Shimokawa, Isao.

In: Experimental Gerontology, Vol. 42, No. 11, 11.2007, p. 1063-1071.

Research output: Contribution to journalArticle

To, K, Yamaza, H, Komatsu, T, Hayashida, T, Hayashi, H, Toyama, H, Chiba, T, Higami, Y & Shimokawa, I 2007, 'Down-regulation of AMP-activated protein kinase by calorie restriction in rat liver', Experimental Gerontology, vol. 42, no. 11, pp. 1063-1071. https://doi.org/10.1016/j.exger.2007.07.003
To, Kazuo ; Yamaza, Haruyoshi ; Komatsu, Toshimitsu ; Hayashida, Takahiro ; Hayashi, Hiroko ; Toyama, Hiroaki ; Chiba, Takuya ; Higami, Yoshikazu ; Shimokawa, Isao. / Down-regulation of AMP-activated protein kinase by calorie restriction in rat liver. In: Experimental Gerontology. 2007 ; Vol. 42, No. 11. pp. 1063-1071.
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