Down-regulation of human galβ1,3GalNAc/galβ1,4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene during differentiation of the HL-60 cell line

Akiyoshi Taniguchi, Yuko Hasegawa, Koji Higai, Kojiro Matsumoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We studied, the regulation of the human Galβ1, 3GalNAc/Galβ1,4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene during HL-60 cell differentiation induced by dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). During differentiation, levels of hST3Gal IV mRNA dramatically decreased after 1 day of stimulation. Reversetranscription PCR identified two mRNA isoforms, types B1 and BX, in HL-60 cells. The results of luciferase assays showed that the level of B3 promoter activity is high, whereas A1/2 and B2 promoter activities are low in HL-60 cells, suggesting that type B1, BX, and B3 mRNA isoforms are expressed in HL-60 cells. A luciferase assay identified a functional DNA portion within the proximal region of the B3 promoter that confers negative transcriptional regulation on the hST3Gal IV B3 promoter during HL-60 differentiation. These results suggest that this element plays a critical role in down-regulating the B3 promoter activity during HL-60 cell differentiation.

Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalMolecular Cell Biology Research Communications
Volume2
Issue number3
Publication statusPublished - 1999 Sep
Externally publishedYes

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Sialyltransferases
HL-60 Cells
Down-Regulation
Cell Line
RNA Isoforms
Genes
Luciferases
Cell Differentiation
Tetradecanoylphorbol Acetate
Dimethyl Sulfoxide
Tretinoin
Genetic Promoter Regions
Polymerase Chain Reaction
Messenger RNA
DNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Down-regulation of human galβ1,3GalNAc/galβ1,4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene during differentiation of the HL-60 cell line. / Taniguchi, Akiyoshi; Hasegawa, Yuko; Higai, Koji; Matsumoto, Kojiro.

In: Molecular Cell Biology Research Communications, Vol. 2, No. 3, 09.1999, p. 197-201.

Research output: Contribution to journalArticle

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abstract = "We studied, the regulation of the human Galβ1, 3GalNAc/Galβ1,4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene during HL-60 cell differentiation induced by dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). During differentiation, levels of hST3Gal IV mRNA dramatically decreased after 1 day of stimulation. Reversetranscription PCR identified two mRNA isoforms, types B1 and BX, in HL-60 cells. The results of luciferase assays showed that the level of B3 promoter activity is high, whereas A1/2 and B2 promoter activities are low in HL-60 cells, suggesting that type B1, BX, and B3 mRNA isoforms are expressed in HL-60 cells. A luciferase assay identified a functional DNA portion within the proximal region of the B3 promoter that confers negative transcriptional regulation on the hST3Gal IV B3 promoter during HL-60 differentiation. These results suggest that this element plays a critical role in down-regulating the B3 promoter activity during HL-60 cell differentiation.",
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