Drift and fluctuating motion of artificial platelet during adhesion process near the wall

H. Tobimatsu, A. Paragon, Y. Okamura, Shinji Takeoka, R. Sudo, K. Tanishita

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    To obtain long trajectories including lateral motion, we built a travelling stage of microscope for tracking particles moving through flow channel and estimated the lateral motion which contributes to NWE and adhesion. We employed recombinant Glycoprotein Ib alpha conjugated latex beads (rGPIbalpha-LB) as platelet substitute. These particles were observed at wall shear rate (WSR) of 200, 500, and 1000 /s with hematocrit of 0 and 40% of washed red blood cells in rectangular flow channel which had vWf surface. We tracked the particle as Lagrangian method and separated the trajectory of particle with drift and fluctuating motion, and investigated quantitatively the motion which contributes to NWE and adhesion to the wall surface. Trajectories of rGPIbalpha-LB were tracked from obtained movies. Then lateral gradient which reflects a drift motion of the particle toward the wall and dispersion coefficient which reflects a fluctuating motion of the particle were calculated. The rGPIbalpha-LB moved only along axial direction with 0% hematocrit. As hematocrit increases, rGPIbalpha-LB moved toward near the wall (about 0.9R) and the position was similar to that of NWE in previous studies. The dispersion coefficient increased near the wall and as WSR and hematocrit increased. In the near wall region, adhered particles showed significantly a tendency toward the wall and high fluctuating motion compared with non-contact particles. We concluded the particle which has drift motion toward the wall with high fluctuating motion induced by the presence of RBC and high shear contributes to interact to the wall surface and adhesion from NWE region.

    Original languageEnglish
    Title of host publicationIFMBE Proceedings
    Pages1075-1078
    Number of pages4
    Volume31 IFMBE
    DOIs
    Publication statusPublished - 2010
    Event6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore
    Duration: 2010 Aug 12010 Aug 6

    Other

    Other6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
    CitySingapore
    Period10/8/110/8/6

    Fingerprint

    Platelet Glycoprotein GPIb-IX Complex
    Glycoproteins
    Latex
    Platelets
    Latexes
    Adhesion
    Trajectories
    Channel flow
    Shear deformation
    Particles (particulate matter)
    Microscopes
    Blood
    Cells

    Keywords

    • Artificial platelet
    • GPIb alpha
    • Near Wall Excess
    • vWf

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Bioengineering

    Cite this

    Tobimatsu, H., Paragon, A., Okamura, Y., Takeoka, S., Sudo, R., & Tanishita, K. (2010). Drift and fluctuating motion of artificial platelet during adhesion process near the wall. In IFMBE Proceedings (Vol. 31 IFMBE, pp. 1075-1078) https://doi.org/10.1007/978-3-642-14515-5_273

    Drift and fluctuating motion of artificial platelet during adhesion process near the wall. / Tobimatsu, H.; Paragon, A.; Okamura, Y.; Takeoka, Shinji; Sudo, R.; Tanishita, K.

    IFMBE Proceedings. Vol. 31 IFMBE 2010. p. 1075-1078.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Tobimatsu, H, Paragon, A, Okamura, Y, Takeoka, S, Sudo, R & Tanishita, K 2010, Drift and fluctuating motion of artificial platelet during adhesion process near the wall. in IFMBE Proceedings. vol. 31 IFMBE, pp. 1075-1078, 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech, Singapore, 10/8/1. https://doi.org/10.1007/978-3-642-14515-5_273
    Tobimatsu H, Paragon A, Okamura Y, Takeoka S, Sudo R, Tanishita K. Drift and fluctuating motion of artificial platelet during adhesion process near the wall. In IFMBE Proceedings. Vol. 31 IFMBE. 2010. p. 1075-1078 https://doi.org/10.1007/978-3-642-14515-5_273
    Tobimatsu, H. ; Paragon, A. ; Okamura, Y. ; Takeoka, Shinji ; Sudo, R. ; Tanishita, K. / Drift and fluctuating motion of artificial platelet during adhesion process near the wall. IFMBE Proceedings. Vol. 31 IFMBE 2010. pp. 1075-1078
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