Drift and fluctuating motion of artificial platelet during adhesion process near the wall

H. Tobimatsu, A. Paragon, Y. Okamura, S. Takeoka, R. Sudo, K. Tanishita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To obtain long trajectories including lateral motion, we built a travelling stage of microscope for tracking particles moving through flow channel and estimated the lateral motion which contributes to NWE and adhesion. We employed recombinant Glycoprotein Ib alpha conjugated latex beads (rGPIbalpha-LB) as platelet substitute. These particles were observed at wall shear rate (WSR) of 200, 500, and 1000 /s with hematocrit of 0 and 40% of washed red blood cells in rectangular flow channel which had vWf surface. We tracked the particle as Lagrangian method and separated the trajectory of particle with drift and fluctuating motion, and investigated quantitatively the motion which contributes to NWE and adhesion to the wall surface. Trajectories of rGPIbalpha-LB were tracked from obtained movies. Then lateral gradient which reflects a drift motion of the particle toward the wall and dispersion coefficient which reflects a fluctuating motion of the particle were calculated. The rGPIbalpha-LB moved only along axial direction with 0% hematocrit. As hematocrit increases, rGPIbalpha-LB moved toward near the wall (about 0.9R) and the position was similar to that of NWE in previous studies. The dispersion coefficient increased near the wall and as WSR and hematocrit increased. In the near wall region, adhered particles showed significantly a tendency toward the wall and high fluctuating motion compared with non-contact particles. We concluded the particle which has drift motion toward the wall with high fluctuating motion induced by the presence of RBC and high shear contributes to interact to the wall surface and adhesion from NWE region.

Original languageEnglish
Title of host publication6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Pages1075-1078
Number of pages4
DOIs
Publication statusPublished - 2010 Oct 22
Event6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore, Singapore
Duration: 2010 Aug 12010 Aug 6

Publication series

NameIFMBE Proceedings
Volume31 IFMBE
ISSN (Print)1680-0737

Conference

Conference6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
CountrySingapore
CitySingapore
Period10/8/110/8/6

Keywords

  • Artificial platelet
  • GPIb alpha
  • Near Wall Excess
  • vWf

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Tobimatsu, H., Paragon, A., Okamura, Y., Takeoka, S., Sudo, R., & Tanishita, K. (2010). Drift and fluctuating motion of artificial platelet during adhesion process near the wall. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech (pp. 1075-1078). (IFMBE Proceedings; Vol. 31 IFMBE). https://doi.org/10.1007/978-3-642-14515-5_273