Droplet microfluidics for precise and high throughput whole genome amplification toward single-cell genome sequencing

Masahito Hosokawa, Y. Nishikawa, Masato Kogawa, Haruko Takeyama

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Single-cell genomics has enabled the exploration of cellular diversity in a broad range of biological samples including tumor and environmental microbes. However, next-generation sequencing (NGS) typically requires nanogram to microgram levels of input DNA. Since single cells contain only a few femtograms (microbe) to picograms (mammalian) of DNA, whole genome amplification (WGA) is required to amplify single-cell genome to adequate amount for sequencing. In addition, high-throughput genome amplification is required to obtain various data from heterogeneous cell populations. To address these problems, we aimed to develop novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction environments for single-cell whole genome amplification.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages178-179
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Keywords

  • Droplet
  • Microfluidics
  • NGS
  • Single cell genomics

ASJC Scopus subject areas

  • Control and Systems Engineering

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