Droplet network connected by biological nanopores for DNA computing

Hiroki Yasuga, R. Kawano, M. Takinoue, Y. Tsuji, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

This paper experimentally describes formation of lipid-coated droplet network and demonstrates a biological NAND logic gate using this network and DNA. Droplets which compose this network are comparted by bilayer lipid membranes (BLMs) and connected via biological nanopores of membrane proteins, which ssDNA passes through. In the geometry of the droplet network, four droplets were placed at the corners and the center of an equilateral triangle. Inputs corresponded to the DNA translocation via biological nanopores from the two droplets at the corners (#1 and 2 in Fig. 1) to the center (#3 in Fig. 1), which can be controlled by the applied electrical fields between the droplets. Output was determined by whether DNA translocation occurred through nanopores between the center (#3 in Fig. 1) and the other corner droplet (#4 in Fig. 1). We believe that this system enables direct connection between electrical systems and computing with biomolecules.

Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII
Subtitle of host publicationThe 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages1221-1222
Number of pages2
DOIs
Publication statusPublished - 2013 Dec 1
Externally publishedYes
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain
Duration: 2013 Jun 162013 Jun 20

Publication series

Name2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Conference

Conference2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
CountrySpain
CityBarcelona
Period13/6/1613/6/20

Fingerprint

Nanopores
DNA
Lipid bilayers
Logic gates
Biomolecules
Lipids
Proteins
Membranes
Geometry

Keywords

  • α-Hemolysin
  • Bilayer Lipid Membrane
  • Biological Nanopore
  • DNA
  • Droplet Contact Method
  • Droplet Network
  • Streptolysin O

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Yasuga, H., Kawano, R., Takinoue, M., Tsuji, Y., Osaki, T., Kamiya, K., ... Takeuchi, S. (2013). Droplet network connected by biological nanopores for DNA computing. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 1221-1222). [6626994] (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). https://doi.org/10.1109/Transducers.2013.6626994

Droplet network connected by biological nanopores for DNA computing. / Yasuga, Hiroki; Kawano, R.; Takinoue, M.; Tsuji, Y.; Osaki, T.; Kamiya, K.; Miki, N.; Takeuchi, S.

2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 1221-1222 6626994 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yasuga, H, Kawano, R, Takinoue, M, Tsuji, Y, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2013, Droplet network connected by biological nanopores for DNA computing. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6626994, 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, pp. 1221-1222, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 13/6/16. https://doi.org/10.1109/Transducers.2013.6626994
Yasuga H, Kawano R, Takinoue M, Tsuji Y, Osaki T, Kamiya K et al. Droplet network connected by biological nanopores for DNA computing. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 1221-1222. 6626994. (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). https://doi.org/10.1109/Transducers.2013.6626994
Yasuga, Hiroki ; Kawano, R. ; Takinoue, M. ; Tsuji, Y. ; Osaki, T. ; Kamiya, K. ; Miki, N. ; Takeuchi, S. / Droplet network connected by biological nanopores for DNA computing. 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. pp. 1221-1222 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).
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