Dual therapeutic action of antibiotic-loaded nanosheets for the treatment of gastrointestinal tissue defects

Toshinori Fujie, Akihiro Saito, Manabu Kinoshita, Hiromi Miyazaki, Shinya Ohtsubo, Daizoh Saitoh, Shinji Takeoka

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

An ultra-thin polymer film (nanosheet) composed of polysaccharides (i.e., polysaccharide nanosheet) provides sufficient adhesiveness, flexibility and robustness to act as an effective wound dressing. We have recently demonstrated the sealing effect of a nanosheet on a murine cecal puncture. Nevertheless, a small percentage of bacteria penetrated the nanosheet because of its ultra-thin structure. Here, we have developed an antibiotic-loaded nanosheet to inhibit bacterial penetration and investigated its therapeutic efficacy using a model of a murine cecal puncture. Tetracycline (TC) was sandwiched between a poly(vinylacetate) (PVAc) layer and the polysaccharide nanosheet (named PVAc-TC-nanosheet). Under physiological conditions, TC was released from the nanosheet for 6 h. Microscopic observation between the interface of the PVAc-TC-nanosheet and bacteria demonstrated how its potent anti-microbial effect was achieved. In vivo studies show that overlapping therapy with the PVAc-TC-nanosheet (thickness: 177 nm) significantly increases mouse survival rate after cecal puncture as well as suppressing an increase in the intraperitoneal bacterial count and leukocyte count.

Original languageEnglish
Pages (from-to)6269-6278
Number of pages10
JournalBiomaterials
Volume31
Issue number24
DOIs
Publication statusPublished - 2010 Aug 1

Keywords

  • Anti-microbial
  • In vivo test
  • Inflammation
  • Nanocomposite
  • Polysaccharide
  • Wound dressing

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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