Dual-view microscopy with a single camera

Real-time imaging of molecular orientations and calcium

Kazuhiko Kinosita, Hiroyasu Itoh, Shin'ichi Ishiwata, Ken'ichi Hirano, Takayuki Nishizaka, Tsuyoshi Hayakawa

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

A new microscope technique, termed "W" (double view video) microscopy, enables simultaneous observation of two different images of an object through a single video camera or by eye. The image pair may, for example, be transmission and fluorescence, fluorescence at different wavelengths, or mutually perpendicular components of polarized fluorescence. Any video microscope can be converted into a dual imager by simple insertion of a small optical device. The continuous appearance of the dual image assures the best time resolution in existing and future video microscopes. As an application, orientations of actin protomers in individual, moving actin filaments have been imaged at the video rate. Asymmetric calcium influxes into a cell exposed to an intense electric pulse have also been visualized.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalJournal of Cell Biology
Volume115
Issue number1
Publication statusPublished - 1991 Oct
Externally publishedYes

Fingerprint

Molecular Imaging
Microscopy
Fluorescence
Calcium
Video Microscopy
Optical Devices
Protein Subunits
Actin Cytoskeleton
Actins
Observation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kinosita, K., Itoh, H., Ishiwata, S., Hirano, K., Nishizaka, T., & Hayakawa, T. (1991). Dual-view microscopy with a single camera: Real-time imaging of molecular orientations and calcium. Journal of Cell Biology, 115(1), 67-73.

Dual-view microscopy with a single camera : Real-time imaging of molecular orientations and calcium. / Kinosita, Kazuhiko; Itoh, Hiroyasu; Ishiwata, Shin'ichi; Hirano, Ken'ichi; Nishizaka, Takayuki; Hayakawa, Tsuyoshi.

In: Journal of Cell Biology, Vol. 115, No. 1, 10.1991, p. 67-73.

Research output: Contribution to journalArticle

Kinosita, K, Itoh, H, Ishiwata, S, Hirano, K, Nishizaka, T & Hayakawa, T 1991, 'Dual-view microscopy with a single camera: Real-time imaging of molecular orientations and calcium', Journal of Cell Biology, vol. 115, no. 1, pp. 67-73.
Kinosita K, Itoh H, Ishiwata S, Hirano K, Nishizaka T, Hayakawa T. Dual-view microscopy with a single camera: Real-time imaging of molecular orientations and calcium. Journal of Cell Biology. 1991 Oct;115(1):67-73.
Kinosita, Kazuhiko ; Itoh, Hiroyasu ; Ishiwata, Shin'ichi ; Hirano, Ken'ichi ; Nishizaka, Takayuki ; Hayakawa, Tsuyoshi. / Dual-view microscopy with a single camera : Real-time imaging of molecular orientations and calcium. In: Journal of Cell Biology. 1991 ; Vol. 115, No. 1. pp. 67-73.
@article{cac7859b27fd42cbb1c0cfd1ad5b1518,
title = "Dual-view microscopy with a single camera: Real-time imaging of molecular orientations and calcium",
abstract = "A new microscope technique, termed {"}W{"} (double view video) microscopy, enables simultaneous observation of two different images of an object through a single video camera or by eye. The image pair may, for example, be transmission and fluorescence, fluorescence at different wavelengths, or mutually perpendicular components of polarized fluorescence. Any video microscope can be converted into a dual imager by simple insertion of a small optical device. The continuous appearance of the dual image assures the best time resolution in existing and future video microscopes. As an application, orientations of actin protomers in individual, moving actin filaments have been imaged at the video rate. Asymmetric calcium influxes into a cell exposed to an intense electric pulse have also been visualized.",
author = "Kazuhiko Kinosita and Hiroyasu Itoh and Shin'ichi Ishiwata and Ken'ichi Hirano and Takayuki Nishizaka and Tsuyoshi Hayakawa",
year = "1991",
month = "10",
language = "English",
volume = "115",
pages = "67--73",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - Dual-view microscopy with a single camera

T2 - Real-time imaging of molecular orientations and calcium

AU - Kinosita, Kazuhiko

AU - Itoh, Hiroyasu

AU - Ishiwata, Shin'ichi

AU - Hirano, Ken'ichi

AU - Nishizaka, Takayuki

AU - Hayakawa, Tsuyoshi

PY - 1991/10

Y1 - 1991/10

N2 - A new microscope technique, termed "W" (double view video) microscopy, enables simultaneous observation of two different images of an object through a single video camera or by eye. The image pair may, for example, be transmission and fluorescence, fluorescence at different wavelengths, or mutually perpendicular components of polarized fluorescence. Any video microscope can be converted into a dual imager by simple insertion of a small optical device. The continuous appearance of the dual image assures the best time resolution in existing and future video microscopes. As an application, orientations of actin protomers in individual, moving actin filaments have been imaged at the video rate. Asymmetric calcium influxes into a cell exposed to an intense electric pulse have also been visualized.

AB - A new microscope technique, termed "W" (double view video) microscopy, enables simultaneous observation of two different images of an object through a single video camera or by eye. The image pair may, for example, be transmission and fluorescence, fluorescence at different wavelengths, or mutually perpendicular components of polarized fluorescence. Any video microscope can be converted into a dual imager by simple insertion of a small optical device. The continuous appearance of the dual image assures the best time resolution in existing and future video microscopes. As an application, orientations of actin protomers in individual, moving actin filaments have been imaged at the video rate. Asymmetric calcium influxes into a cell exposed to an intense electric pulse have also been visualized.

UR - http://www.scopus.com/inward/record.url?scp=0025992141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025992141&partnerID=8YFLogxK

M3 - Article

VL - 115

SP - 67

EP - 73

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

ER -