TY - JOUR
T1 - Dynamic analysis of a genomic island in Magnetospirillum sp. strain AMB-1 reveals how magnetosome synthesis developed
AU - Fukuda, Yorikane
AU - Okamura, Yoshiko
AU - Takeyama, Haruko
AU - Matsunaga, Tadashi
N1 - Funding Information:
This work was funded in part by Grant-in-Aid for Specially Promoted Research, No. 13002005 from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2006/2/6
Y1 - 2006/2/6
N2 - The entire structure of a 98 kb genomic region that abounds in genes related to magnetosome synthesis was first described in the Magnetospirillum sp. strain AMB-1. The deletion of this 98 kb genomic region and the circular form after excision from the chromosome was detected by PCR amplification. This strongly suggests that the region has undergone a lateral gene transfer. The region has the characteristics of a genomic island: low GC content, location between two repetitive sequences, and the presence of an integrase in the flanking region of the first repetitive sequence. This 98 kb genomic region has the potential for transfer by the integrase activity. Comparative genome analysis revealed other regions with a high concentration of orthologs in magnetic bacteria besides the 98 kb region, and magnetosome synthesis seemed to need not only the exogenous 98 kb region, but also other orthologs and individually originating genes.
AB - The entire structure of a 98 kb genomic region that abounds in genes related to magnetosome synthesis was first described in the Magnetospirillum sp. strain AMB-1. The deletion of this 98 kb genomic region and the circular form after excision from the chromosome was detected by PCR amplification. This strongly suggests that the region has undergone a lateral gene transfer. The region has the characteristics of a genomic island: low GC content, location between two repetitive sequences, and the presence of an integrase in the flanking region of the first repetitive sequence. This 98 kb genomic region has the potential for transfer by the integrase activity. Comparative genome analysis revealed other regions with a high concentration of orthologs in magnetic bacteria besides the 98 kb region, and magnetosome synthesis seemed to need not only the exogenous 98 kb region, but also other orthologs and individually originating genes.
KW - Comparative analysis
KW - Genomic island
KW - Magnetic bacteria
KW - Magnetosome synthesis
KW - Magnetospirillum sp. strain AMB-1
UR - http://www.scopus.com/inward/record.url?scp=31444443960&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31444443960&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2006.01.003
DO - 10.1016/j.febslet.2006.01.003
M3 - Article
C2 - 16423350
AN - SCOPUS:31444443960
SN - 0014-5793
VL - 580
SP - 801
EP - 812
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -