Dynamic light scattering study of muscle f-actin

Satoru Fujime, Shin'ichi Ishiwata, Tadakazu Maeda

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    By use of digital autocorrelation and fast Fourier methods, dynamic light-scattering studies of in vitro reconstituted muscle F-actin were made over a wide range of concentrations. 0.01-2 mg ml F-actin. Measurements of correlation function [g1(t)]2 showed that a transition from a dilute to a semidilute regime for the Brownian motions of filaments occurred at around 0.3 mg ml F-actin. Beyond this concentration, profiles of successively measured [g1(t)]2 showed very poor reproducibility. This resulted from the existence of very slow components, which could not be measured with a high statistical accuracy even for a measuring time of 3600 s/run. On the other hand, subtraction of these components automatically by an electronic circuit, [g-1(t)]2, or by computer processing, [g1(t)]2, resulted in a fairly good reproducibility of the profies, The decay c of [g-1(t)]2 (and [g-1(t)]2) were very similar to those of [g1(t)]2 for dilute solutions. A theoret which could account for the above situation. The time sequence {n(t,T)} of photoelectron counts at a sampling time T of light scattered from semidilute solutions of F-actin was stored on magnetic tapes, and both power spectra S̄(f) and correlation functions [g-1(t)]2 were computed by taking the ensemble average over many short records with duration 1024T. Since both S̄(f and [g-1(t)]2 lacked frequency components lower than 1 (2048T) Hz. their profiles were highly reproducible. An of S̄(f) confirmed our earlier results which had shown an apparent contradiction to later results by a correlation method. A comparison of S̄(f) and [g-1(t)]2 based on the same {n(t,T)} clarified the reasons why the bandwidth Γ of S̄( differed from the bandwidth \ ̄gG of [g1(t)]2 and [g-1(t)]2. The temperature dependence of Γ suggested that F-actin flexible and that the flexibility parameter would change with temperature.

    Original languageEnglish
    Pages (from-to)1-21
    Number of pages21
    JournalBiophysical Chemistry
    Volume20
    Issue number1-2
    DOIs
    Publication statusPublished - 1984

    Fingerprint

    Dynamic light scattering
    muscles
    Muscle
    Actins
    light scattering
    Muscles
    bandwidth
    magnetic tapes
    Bandwidth
    profiles
    Magnetic tape
    subtraction
    Temperature
    Correlation methods
    Brownian movement
    autocorrelation
    power spectra
    filaments
    flexibility
    Photoelectrons

    Keywords

    • Dynamic light scattering
    • F-Actin
    • Fast Fourier method
    • Filament flexibility
    • Semidilute solution

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Physical and Theoretical Chemistry

    Cite this

    Dynamic light scattering study of muscle f-actin. / Fujime, Satoru; Ishiwata, Shin'ichi; Maeda, Tadakazu.

    In: Biophysical Chemistry, Vol. 20, No. 1-2, 1984, p. 1-21.

    Research output: Contribution to journalArticle

    Fujime, Satoru ; Ishiwata, Shin'ichi ; Maeda, Tadakazu. / Dynamic light scattering study of muscle f-actin. In: Biophysical Chemistry. 1984 ; Vol. 20, No. 1-2. pp. 1-21.
    @article{dce2a03708214c8bba5c638ddd5fab39,
    title = "Dynamic light scattering study of muscle f-actin",
    abstract = "By use of digital autocorrelation and fast Fourier methods, dynamic light-scattering studies of in vitro reconstituted muscle F-actin were made over a wide range of concentrations. 0.01-2 mg ml F-actin. Measurements of correlation function [g1(t)]2 showed that a transition from a dilute to a semidilute regime for the Brownian motions of filaments occurred at around 0.3 mg ml F-actin. Beyond this concentration, profiles of successively measured [g1(t)]2 showed very poor reproducibility. This resulted from the existence of very slow components, which could not be measured with a high statistical accuracy even for a measuring time of 3600 s/run. On the other hand, subtraction of these components automatically by an electronic circuit, [g-1(t)]2, or by computer processing, [g1(t)]2, resulted in a fairly good reproducibility of the profies, The decay c of [g-1(t)]2 (and [g-1(t)]2) were very similar to those of [g1(t)]2 for dilute solutions. A theoret which could account for the above situation. The time sequence {n(t,T)} of photoelectron counts at a sampling time T of light scattered from semidilute solutions of F-actin was stored on magnetic tapes, and both power spectra S̄(f) and correlation functions [g-1(t)]2 were computed by taking the ensemble average over many short records with duration 1024T. Since both S̄(f and [g-1(t)]2 lacked frequency components lower than 1 (2048T) Hz. their profiles were highly reproducible. An of S̄(f) confirmed our earlier results which had shown an apparent contradiction to later results by a correlation method. A comparison of S̄(f) and [g-1(t)]2 based on the same {n(t,T)} clarified the reasons why the bandwidth Γ of S̄( differed from the bandwidth \ ̄gG of [g1(t)]2 and [g-1(t)]2. The temperature dependence of Γ suggested that F-actin flexible and that the flexibility parameter would change with temperature.",
    keywords = "Dynamic light scattering, F-Actin, Fast Fourier method, Filament flexibility, Semidilute solution",
    author = "Satoru Fujime and Shin'ichi Ishiwata and Tadakazu Maeda",
    year = "1984",
    doi = "10.1016/0301-4622(84)80001-0",
    language = "English",
    volume = "20",
    pages = "1--21",
    journal = "Biophysical Chemistry",
    issn = "0301-4622",
    publisher = "Elsevier",
    number = "1-2",

    }

    TY - JOUR

    T1 - Dynamic light scattering study of muscle f-actin

    AU - Fujime, Satoru

    AU - Ishiwata, Shin'ichi

    AU - Maeda, Tadakazu

    PY - 1984

    Y1 - 1984

    N2 - By use of digital autocorrelation and fast Fourier methods, dynamic light-scattering studies of in vitro reconstituted muscle F-actin were made over a wide range of concentrations. 0.01-2 mg ml F-actin. Measurements of correlation function [g1(t)]2 showed that a transition from a dilute to a semidilute regime for the Brownian motions of filaments occurred at around 0.3 mg ml F-actin. Beyond this concentration, profiles of successively measured [g1(t)]2 showed very poor reproducibility. This resulted from the existence of very slow components, which could not be measured with a high statistical accuracy even for a measuring time of 3600 s/run. On the other hand, subtraction of these components automatically by an electronic circuit, [g-1(t)]2, or by computer processing, [g1(t)]2, resulted in a fairly good reproducibility of the profies, The decay c of [g-1(t)]2 (and [g-1(t)]2) were very similar to those of [g1(t)]2 for dilute solutions. A theoret which could account for the above situation. The time sequence {n(t,T)} of photoelectron counts at a sampling time T of light scattered from semidilute solutions of F-actin was stored on magnetic tapes, and both power spectra S̄(f) and correlation functions [g-1(t)]2 were computed by taking the ensemble average over many short records with duration 1024T. Since both S̄(f and [g-1(t)]2 lacked frequency components lower than 1 (2048T) Hz. their profiles were highly reproducible. An of S̄(f) confirmed our earlier results which had shown an apparent contradiction to later results by a correlation method. A comparison of S̄(f) and [g-1(t)]2 based on the same {n(t,T)} clarified the reasons why the bandwidth Γ of S̄( differed from the bandwidth \ ̄gG of [g1(t)]2 and [g-1(t)]2. The temperature dependence of Γ suggested that F-actin flexible and that the flexibility parameter would change with temperature.

    AB - By use of digital autocorrelation and fast Fourier methods, dynamic light-scattering studies of in vitro reconstituted muscle F-actin were made over a wide range of concentrations. 0.01-2 mg ml F-actin. Measurements of correlation function [g1(t)]2 showed that a transition from a dilute to a semidilute regime for the Brownian motions of filaments occurred at around 0.3 mg ml F-actin. Beyond this concentration, profiles of successively measured [g1(t)]2 showed very poor reproducibility. This resulted from the existence of very slow components, which could not be measured with a high statistical accuracy even for a measuring time of 3600 s/run. On the other hand, subtraction of these components automatically by an electronic circuit, [g-1(t)]2, or by computer processing, [g1(t)]2, resulted in a fairly good reproducibility of the profies, The decay c of [g-1(t)]2 (and [g-1(t)]2) were very similar to those of [g1(t)]2 for dilute solutions. A theoret which could account for the above situation. The time sequence {n(t,T)} of photoelectron counts at a sampling time T of light scattered from semidilute solutions of F-actin was stored on magnetic tapes, and both power spectra S̄(f) and correlation functions [g-1(t)]2 were computed by taking the ensemble average over many short records with duration 1024T. Since both S̄(f and [g-1(t)]2 lacked frequency components lower than 1 (2048T) Hz. their profiles were highly reproducible. An of S̄(f) confirmed our earlier results which had shown an apparent contradiction to later results by a correlation method. A comparison of S̄(f) and [g-1(t)]2 based on the same {n(t,T)} clarified the reasons why the bandwidth Γ of S̄( differed from the bandwidth \ ̄gG of [g1(t)]2 and [g-1(t)]2. The temperature dependence of Γ suggested that F-actin flexible and that the flexibility parameter would change with temperature.

    KW - Dynamic light scattering

    KW - F-Actin

    KW - Fast Fourier method

    KW - Filament flexibility

    KW - Semidilute solution

    UR - http://www.scopus.com/inward/record.url?scp=0021188467&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0021188467&partnerID=8YFLogxK

    U2 - 10.1016/0301-4622(84)80001-0

    DO - 10.1016/0301-4622(84)80001-0

    M3 - Article

    C2 - 6487741

    AN - SCOPUS:0021188467

    VL - 20

    SP - 1

    EP - 21

    JO - Biophysical Chemistry

    JF - Biophysical Chemistry

    SN - 0301-4622

    IS - 1-2

    ER -