Dynamic light scattering study of pig vitreous body

Toyoaki Matsuura, Naokazu Idota, Yoshiaki Hara, Masahiko Annaka

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The phase behaviors and dynamical properties of pig vitreous body were studied by macroscopic observation of swelling behavior and dynamic light scattering under various conditions. From the observations of the dynamics of light scattered by the pig vitreous body under physiological condition, intensity autocorrelation functions that revealed two diffusion coefficients, Dfast and Dslow were obtained. We developed the theory for describing the density fluctuation of the entities in the vitreous gel system with sodium hyaluronate filled in the meshes of collagen fiber network. The dynamics of collagen and sodium hyaluronate explains two relaxation modes of the fluctuation. The diffusion coefficient of collagen obtained from Dfast and Dslow is very close to that in aqueous solution, which suggests the vitreous body is in the swollen state. Divergent behavior in the measured total scattered light intensities and diffusion coefficients upon varying the concentration of salt (NaCl and CaCl2) was observed. Namely, a slowing down of the dynamic modes accompanied by increased "static" scattered intensities was observed. This is indicative of the occurrence of a phase transition upon salt concentration.

Original languageEnglish
Title of host publicationProgress in Colloid and Polymer Science
Pages195-204
Number of pages10
Volume136
DOIs
Publication statusPublished - 2009
Externally publishedYes

Publication series

NameProgress in Colloid and Polymer Science
Volume136
ISSN (Print)0340255X

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Keywords

  • Collagen
  • Critical phenomenon
  • Dynamic light scattering
  • Phase transition
  • Sodium hyaluronate
  • Vitreous body

ASJC Scopus subject areas

  • Organic Chemistry
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Matsuura, T., Idota, N., Hara, Y., & Annaka, M. (2009). Dynamic light scattering study of pig vitreous body. In Progress in Colloid and Polymer Science (Vol. 136, pp. 195-204). (Progress in Colloid and Polymer Science; Vol. 136). https://doi.org/10.1007/2882_2009_27