Dynamic methylation pattern of the methyltransferase1o (Dnmt1o) 5′-flanking region during mouse oogenesis and spermatogenesis

Yeoung Gyu Ko, Jisoo Yun, Hyoung Joon Park, Satoshi Tanaka, Kunio Shiota, Jae Hyeon Cho

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

DNA methyltransferase1o (Dnmt1o), which is specific to oocyte and preimplantation embryo, plays a role in maintaining DNA methylation in mammalian cells. Here, we investigated the methylation status of CpGs sites in the Dnmt1o 5′-flanking region in germ cells at different stages of oogenesis or spermatogenesis. The methylation levels of the CpG sites at the 5′-flanking regions were hypermethylated in growing oocytes of all follicular stages, while the oocytes in meiotic metaphase II (MII) were demethylated. The methylation pattern within the CpGs sites in the 5′-flanking region, however, was dramatically changed during spermatogenesis. We observed that there was significant non-CpG methylation both in MII oocytes and spermatocytes. Although a low methylation level in non-CpG sites was observed in primary and secondary oocytes, the CpA site of position 25 and CpT site of position 29 within the no-CpG region in the 5′-flanking region of Dnmt1o was highly methylated in MII oocytes. During spermatogenesis, the low degree of methylation at CpG sites in spermatocytes increased to a higher degree in sperm, while the high ratio of methylation in non-CpG sites in spermatocytes decreased. Together, germ cells showed inverted methylation patterns between CpG and non-CpG sites in the Dnmt1o 5′-upstream region, and the methylation pattern during oogenesis did not drastically change, remaining generally hypomethylated at the MII stage.

Original languageEnglish
Pages (from-to)212-222
Number of pages11
JournalMolecular Reproduction and Development
Volume80
Issue number3
DOIs
Publication statusPublished - 2013 Mar
Externally publishedYes

Fingerprint

Oogenesis
5' Flanking Region
Spermatogenesis
Methylation
Oocytes
DNA
Metaphase
Spermatocytes
Germ Cells
Blastocyst
DNA Methylation
Spermatozoa

Keywords

  • DNA methylation
  • Methyltransferase1o (Dnmt1o)
  • Oogenesis
  • Spermatogenesis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Dynamic methylation pattern of the methyltransferase1o (Dnmt1o) 5′-flanking region during mouse oogenesis and spermatogenesis. / Ko, Yeoung Gyu; Yun, Jisoo; Park, Hyoung Joon; Tanaka, Satoshi; Shiota, Kunio; Cho, Jae Hyeon.

In: Molecular Reproduction and Development, Vol. 80, No. 3, 03.2013, p. 212-222.

Research output: Contribution to journalArticle

Ko, Yeoung Gyu ; Yun, Jisoo ; Park, Hyoung Joon ; Tanaka, Satoshi ; Shiota, Kunio ; Cho, Jae Hyeon. / Dynamic methylation pattern of the methyltransferase1o (Dnmt1o) 5′-flanking region during mouse oogenesis and spermatogenesis. In: Molecular Reproduction and Development. 2013 ; Vol. 80, No. 3. pp. 212-222.
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