Dynamics of Ca2+ and Na+ in the dendrites of mouse cerebellar Purkinje cells evoked by parallel fibre stimulation

Akinori Kuruma, Takafumi Inoue, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Ca2+ and Na+ play important roles in neurons, such as in synaptic plasticity. Their concentrations in neurons change dynamically in response to synaptic inputs, but their kinetics have not been compared directly. Here, we show the mechanisms and dynamics of Ca2+ and Na+ transients by simultaneous monitoring in Purkinje cell dendrites in mouse cerebellar slices. High frequency parallel fibre stimulation (50 Hz, 3-50-times) depolarized Purkinje cells, and Ca2+ transients were observed at the anatomically expected sites. The magnitude of the Ca 2+ transients increased linearly with increasing numbers of parallel fibre inputs. With 50 stimuli, Ca2+ transients lasted for seconds, and the peak [Ca2+] reached ∼100 μM, which was much higher than that reported previously, although it was still confined to a part of the dendrite. In contrast, Na+ transients were sustained for tens of seconds and diffused away from the stimulated site. Pharmacological interventions revealed that Na+ influx through α-amino-3- hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors and Ca2+ influx through P-type Ca channels were essential players, that AMPA receptors did not operate as a Ca2+ influx pathway and that Ca2+ release from intracellular stores through inositol trisphosphate receptors or ryanodine receptors did not contribute greatly to the large Ca2+ transients.

Original languageEnglish
Pages (from-to)2677-2689
Number of pages13
JournalEuropean Journal of Neuroscience
Volume18
Issue number10
DOIs
Publication statusPublished - 2003 Nov
Externally publishedYes

Fingerprint

AMPA Receptors
Purkinje Cells
Dendrites
Neurons
Ryanodine Receptor Calcium Release Channel
Neuronal Plasticity
Inositol
Pharmacology
Acids

Keywords

  • Calcium dynamics
  • Calcium imaging
  • Glutamate receptor
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dynamics of Ca2+ and Na+ in the dendrites of mouse cerebellar Purkinje cells evoked by parallel fibre stimulation. / Kuruma, Akinori; Inoue, Takafumi; Mikoshiba, Katsuhiko.

In: European Journal of Neuroscience, Vol. 18, No. 10, 11.2003, p. 2677-2689.

Research output: Contribution to journalArticle

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