Dynamics of DNA in chromatin and DNA binding mode to core protein

Ikuo Ashikawa, Kazuhiko Kinosita, Akira Ikegami, Yoshifumi Nishimura, Masamichi Tsuboi, Kazutada Watanabe, Koujiro Iso

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have studied the dynamics of DNA in nucleosome core particles and in the linker region of chromatin using nanosecond fluorescence anisotropy decay measurements of intercalated ethidium. DNA in the core undergoes torsional motions to the same extent as the linker DNA in extended chromatin. We therefore concluded that the binding of DNA to the histone octamer is relatively weak or limited to a few points; stretches of at least several tens of base pairs exist which can move as freely as DNA in solution.

Original languageEnglish
Pages (from-to)665-668
Number of pages4
JournalJournal of Biochemistry
Volume93
Issue number2
Publication statusPublished - 1983 Feb
Externally publishedYes

Fingerprint

Chromatin
DNA
Proteins
Protein
Stretch
Fluorescence
Anisotropy
Decay
Motion
Fluorescence Polarization
Ethidium
Nucleosomes
Base Pairing
Histones

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Ashikawa, I., Kinosita, K., Ikegami, A., Nishimura, Y., Tsuboi, M., Watanabe, K., & Iso, K. (1983). Dynamics of DNA in chromatin and DNA binding mode to core protein. Journal of Biochemistry, 93(2), 665-668.

Dynamics of DNA in chromatin and DNA binding mode to core protein. / Ashikawa, Ikuo; Kinosita, Kazuhiko; Ikegami, Akira; Nishimura, Yoshifumi; Tsuboi, Masamichi; Watanabe, Kazutada; Iso, Koujiro.

In: Journal of Biochemistry, Vol. 93, No. 2, 02.1983, p. 665-668.

Research output: Contribution to journalArticle

Ashikawa, I, Kinosita, K, Ikegami, A, Nishimura, Y, Tsuboi, M, Watanabe, K & Iso, K 1983, 'Dynamics of DNA in chromatin and DNA binding mode to core protein', Journal of Biochemistry, vol. 93, no. 2, pp. 665-668.
Ashikawa I, Kinosita K, Ikegami A, Nishimura Y, Tsuboi M, Watanabe K et al. Dynamics of DNA in chromatin and DNA binding mode to core protein. Journal of Biochemistry. 1983 Feb;93(2):665-668.
Ashikawa, Ikuo ; Kinosita, Kazuhiko ; Ikegami, Akira ; Nishimura, Yoshifumi ; Tsuboi, Masamichi ; Watanabe, Kazutada ; Iso, Koujiro. / Dynamics of DNA in chromatin and DNA binding mode to core protein. In: Journal of Biochemistry. 1983 ; Vol. 93, No. 2. pp. 665-668.
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