Dynamics of DNA in chromatin and DNA binding mode to core protein

Ikuo Ashikawa; Kazuhiko Kinosita; Akira Ikegami; Yoshifumi Nishimura; Masamichi Tsuboi; Kazutada Watanabe; Koujiro Iso

Dynamics of DNA in chromatin and DNA binding mode to core protein

Ikuo Ashikawa, Kazuhiko Kinosita, Akira Ikegami, Yoshifumi Nishimura, Masamichi Tsuboi, Kazutada Watanabe, Koujiro Iso

Research output: Contribution to journal › Article › peer-review

Abstract

We have studied the dynamics of DNA in nucleosome core particles and in the linker region of chromatin using nanosecond fluorescence anisotropy decay measurements of intercalated ethidium. DNA in the core undergoes torsional motions to the same extent as the linker DNA in extended chromatin. We therefore concluded that the binding of DNA to the histone octamer is relatively weak or limited to a few points; stretches of at least several tens of base pairs exist which can move as freely as DNA in solution.

Original language	English
Pages (from-to)	665-668
Number of pages	4
Journal	Journal of Biochemistry
Volume	93
Issue number	2
Publication status	Published - 1983 Feb
Externally published	Yes

ASJC Scopus subject areas

Statistics, Probability and Uncertainty
Applied Mathematics
Physiology (medical)
Radiology Nuclear Medicine and imaging
Molecular Biology
Biochemistry

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author = "Ikuo Ashikawa and Kazuhiko Kinosita and Akira Ikegami and Yoshifumi Nishimura and Masamichi Tsuboi and Kazutada Watanabe and Koujiro Iso",

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AU - Ashikawa, Ikuo

AU - Kinosita, Kazuhiko

AU - Ikegami, Akira

AU - Nishimura, Yoshifumi

AU - Tsuboi, Masamichi

AU - Watanabe, Kazutada

AU - Iso, Koujiro

PY - 1983/2

Y1 - 1983/2

N2 - We have studied the dynamics of DNA in nucleosome core particles and in the linker region of chromatin using nanosecond fluorescence anisotropy decay measurements of intercalated ethidium. DNA in the core undergoes torsional motions to the same extent as the linker DNA in extended chromatin. We therefore concluded that the binding of DNA to the histone octamer is relatively weak or limited to a few points; stretches of at least several tens of base pairs exist which can move as freely as DNA in solution.

AB - We have studied the dynamics of DNA in nucleosome core particles and in the linker region of chromatin using nanosecond fluorescence anisotropy decay measurements of intercalated ethidium. DNA in the core undergoes torsional motions to the same extent as the linker DNA in extended chromatin. We therefore concluded that the binding of DNA to the histone octamer is relatively weak or limited to a few points; stretches of at least several tens of base pairs exist which can move as freely as DNA in solution.

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Dynamics of DNA in chromatin and DNA binding mode to core protein

Abstract

ASJC Scopus subject areas

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