Dynamics of yeast prion aggregates in single living cells

Shigeko Kawai-noma, Satoru Ayano, Chan Gi Pack, Masataka Kinjo, Masasuke Yoshida, Kenji Yasuda, Hideki Taguchi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Prions are propagating proteins that are ordered protein aggregates, in which the phenotypic trait is retained in the altered protein conformers. To understand the dynamics of the prion aggregates in living cells, we directly monitored the fate of the aggregates using an on-chip single-cell cultivation system as well as fluorescence correlation spectroscopy (FCS). Single-cell imaging revealed that the visible foci of yeast prion Sup35 fused with GFP are dispersed throughout the cytoplasm during cell growth, but retain the prion phenotype. FCS showed that [PSI+] cells, irrespective of the presence of foci, contain diffuse oligomers, which are transmitted to their daughter cells. Single-cell observations of the oligomer-based transmission provide a link between previous in vivo and in vitro analyses of the prion and shed light on the relationship between the protein conformation and the phenotype.

Original languageEnglish
Pages (from-to)1085-1096
Number of pages12
JournalGenes to Cells
Volume11
Issue number9
DOIs
Publication statusPublished - 2006 Sep
Externally publishedYes

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Prions
Yeasts
Fluorescence Spectrometry
Phenotype
Protein Conformation
Cytoplasm
Growth
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Kawai-noma, S., Ayano, S., Pack, C. G., Kinjo, M., Yoshida, M., Yasuda, K., & Taguchi, H. (2006). Dynamics of yeast prion aggregates in single living cells. Genes to Cells, 11(9), 1085-1096. https://doi.org/10.1111/j.1365-2443.2006.01004.x

Dynamics of yeast prion aggregates in single living cells. / Kawai-noma, Shigeko; Ayano, Satoru; Pack, Chan Gi; Kinjo, Masataka; Yoshida, Masasuke; Yasuda, Kenji; Taguchi, Hideki.

In: Genes to Cells, Vol. 11, No. 9, 09.2006, p. 1085-1096.

Research output: Contribution to journalArticle

Kawai-noma, S, Ayano, S, Pack, CG, Kinjo, M, Yoshida, M, Yasuda, K & Taguchi, H 2006, 'Dynamics of yeast prion aggregates in single living cells', Genes to Cells, vol. 11, no. 9, pp. 1085-1096. https://doi.org/10.1111/j.1365-2443.2006.01004.x
Kawai-noma S, Ayano S, Pack CG, Kinjo M, Yoshida M, Yasuda K et al. Dynamics of yeast prion aggregates in single living cells. Genes to Cells. 2006 Sep;11(9):1085-1096. https://doi.org/10.1111/j.1365-2443.2006.01004.x
Kawai-noma, Shigeko ; Ayano, Satoru ; Pack, Chan Gi ; Kinjo, Masataka ; Yoshida, Masasuke ; Yasuda, Kenji ; Taguchi, Hideki. / Dynamics of yeast prion aggregates in single living cells. In: Genes to Cells. 2006 ; Vol. 11, No. 9. pp. 1085-1096.
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