Abstract
The torsional and bending rigidities of Z-form DNA have been studied by nanosecond fluorescence anisotropy measurements of intercalated ethidium. The results suggested that Z-form DNA was considerably more flexible than B-form DNA. We have investigated the temperature dependence of the rigidity of B- and Z-form DNA and found that the temperature dependence of the torsional rigidity of Z-form DNA was remarkably lower than that of B-form DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 87-93 |
| Number of pages | 7 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 782 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1984 May 15 |
| Externally published | Yes |
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Keywords
- DNA dynamics
- Ethidium bromide
- Fluorescence anisotropy
- Helix rigidity
- Z-DNA
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Genetics
- Structural Biology
Cite this
Dynamics of Z-form DNA. / Ashikawa, Ikuo; Kinosita, Kazuhiko; Ikegami, Akira.
In: BBA - Gene Structure and Expression, Vol. 782, No. 1, 15.05.1984, p. 87-93.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamics of Z-form DNA
AU - Ashikawa, Ikuo
AU - Kinosita, Kazuhiko
AU - Ikegami, Akira
PY - 1984/5/15
Y1 - 1984/5/15
N2 - The torsional and bending rigidities of Z-form DNA have been studied by nanosecond fluorescence anisotropy measurements of intercalated ethidium. The results suggested that Z-form DNA was considerably more flexible than B-form DNA. We have investigated the temperature dependence of the rigidity of B- and Z-form DNA and found that the temperature dependence of the torsional rigidity of Z-form DNA was remarkably lower than that of B-form DNA.
AB - The torsional and bending rigidities of Z-form DNA have been studied by nanosecond fluorescence anisotropy measurements of intercalated ethidium. The results suggested that Z-form DNA was considerably more flexible than B-form DNA. We have investigated the temperature dependence of the rigidity of B- and Z-form DNA and found that the temperature dependence of the torsional rigidity of Z-form DNA was remarkably lower than that of B-form DNA.
KW - DNA dynamics
KW - Ethidium bromide
KW - Fluorescence anisotropy
KW - Helix rigidity
KW - Z-DNA
UR - http://www.scopus.com/inward/record.url?scp=0021361487&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021361487&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(84)90109-X
DO - 10.1016/0167-4781(84)90109-X
M3 - Article
C2 - 6722160
AN - SCOPUS:0021361487
VL - 782
SP - 87
EP - 93
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -