Dysfunction of the fusion of pre-synaptic plasma membranes and synaptic vesicles caused by oxidative stress, and its prevention by vitamin e

Masaya Arai, Mitsunari Saito, Hirokatsu Takatsu, Koji Fukui, Shiro Urano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To define whether hyperoxia induces the dysfunction of membrane fusion between synaptic vesicles with pre-synaptic plasma membranes in the nerve terminals, and whether vitamin E prevents this abnormal event, we investigated the influence of hyperoxia on the fusion ability of isolated synaptic vesicles and the inside-out type pre-synaptic plasma membrane vesicles from rat brain using the fluorescence tracing method. The membrane fusion ability of both membranes from rats subjected to hyperoxia was markedly decreased compared with the membranes from a normal rat. Rats subjected to hyperoxia in the form of oxidative stress showed significant increases in the levels of thiobarbituric acid reactive substances (TBARS), conjugated dienes, and protein carbonyl moieties in both synaptic vesicles and pre-synaptic plasma membranes. When rats were supplemented with vitamin E, these abnormalities were inhibited even when rats were subjected to hyperoxia.

Original languageEnglish
Pages (from-to)759-766
Number of pages8
JournalJournal of Alzheimer's Disease
Volume24
Issue number4
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Synaptic Membranes
Synaptic Vesicles
Hyperoxia
Vitamins
Oxidative Stress
Cell Membrane
Membrane Fusion
Vitamin E
Membranes
Thiobarbituric Acid Reactive Substances
Fluorescence
Brain
Proteins

Keywords

  • Neurotransmission
  • oxidative brain damage
  • oxidative stress
  • synaptic membrane fusion
  • vitamin E

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Geriatrics and Gerontology
  • Clinical Psychology

Cite this

Dysfunction of the fusion of pre-synaptic plasma membranes and synaptic vesicles caused by oxidative stress, and its prevention by vitamin e. / Arai, Masaya; Saito, Mitsunari; Takatsu, Hirokatsu; Fukui, Koji; Urano, Shiro.

In: Journal of Alzheimer's Disease, Vol. 24, No. 4, 2011, p. 759-766.

Research output: Contribution to journalArticle

Arai, Masaya ; Saito, Mitsunari ; Takatsu, Hirokatsu ; Fukui, Koji ; Urano, Shiro. / Dysfunction of the fusion of pre-synaptic plasma membranes and synaptic vesicles caused by oxidative stress, and its prevention by vitamin e. In: Journal of Alzheimer's Disease. 2011 ; Vol. 24, No. 4. pp. 759-766.
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