Early deprivation induces competitive subordinance in C57BL/6 male mice

Seico Benner, Toshihiro Endo, Nozomi Endo, Masaki Kakeyama, Chiharu Tohyama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Rodent models have been widely used to investigate the impact of early life stress on adult health and behavior. However, the social dimension has rarely been incorporated into the analysis due to methodological limitations. This study characterized the effects of neonatal social isolation (early deprivation, ED) on adult C57BL/6 mouse behavior in a social context using our recently developed behavioral test protocols for group-housed mice. During the first two postnatal weeks, half of the pups per dam were separated from their dam and littermates for 3. h per day (ED group). Post weaning, ED and control pups were electronically tagged and co-housed. At 12. weeks, the mixed cohorts were transferred to IntelliCages, equipped with computer-controlled operant chambers. Access to the chambers was used as an index to analyze novel object response, behavioral flexibility, and competitive dominance with minimal experimenter intervention. In general, ED had greater effects on males; ED males exhibited reduced body weight, increased novelty response, and were subordinate to control littermates when competing for reward access. Male ED mice also demonstrated mildly impaired reversal learning. Analyzing gene expression changes in brain regions controlling emotion, stress, spatial memory, and executive function revealed reduced BDNF and c-Fos in hippocampal CA1, enhanced c-Fos in the basolateral amygdala, reduced Map2 while enhanced HSD11β2 in prefrontal cortex of ED males. In male mice, it was suggested that neonatal social isolation results in sustained changes in social behavior with altered function of limbic and frontal cortices.

Original languageEnglish
Pages (from-to)42-52
Number of pages11
JournalPhysiology and Behavior
Volume137
DOIs
Publication statusPublished - 2014 Oct 2
Externally publishedYes

Fingerprint

Social Isolation
Reversal Learning
Social Behavior
Brain-Derived Neurotrophic Factor
Executive Function
Health Behavior
Frontal Lobe
Weaning
Prefrontal Cortex
Reward
Inbred C57BL Mouse
Psychological Stress
Rodentia
Emotions
Body Weight
Mouse
Deprivation
Gene Expression
Brain
Isolation

Keywords

  • Competitive dominance
  • Early deprivation
  • Early life stress

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Experimental and Cognitive Psychology
  • Philosophy
  • Medicine(all)

Cite this

Early deprivation induces competitive subordinance in C57BL/6 male mice. / Benner, Seico; Endo, Toshihiro; Endo, Nozomi; Kakeyama, Masaki; Tohyama, Chiharu.

In: Physiology and Behavior, Vol. 137, 02.10.2014, p. 42-52.

Research output: Contribution to journalArticle

Benner, Seico ; Endo, Toshihiro ; Endo, Nozomi ; Kakeyama, Masaki ; Tohyama, Chiharu. / Early deprivation induces competitive subordinance in C57BL/6 male mice. In: Physiology and Behavior. 2014 ; Vol. 137. pp. 42-52.
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