The addition of 2-deoxy-d-glucose and related glucose analogues to the cultures of Coprinus macrorhizus resulted in the induction of tyrosinase production and inhibition of fruiting body formation. The induction was inhibited by cycloheximide or high concentrations of glucose. Electrophoretic analyses revealed that tyrosinase, peroxidase, and tyrosinase inhibitors were present in crude mycelial extracts and that the production of an unusual tyrosinase isozyme was responsible for the enzyme induction.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology