Effect of bacterial stress on hydrogen fermentation microflora

Hiroyuki Kashima, Hiroshi Nyunoya, Michiei Oto, Seishu Tojo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we investigated effects of bacterial stress treatment on domination of hydrogen producing bacteria. As the bacterial stress treatment to control hydrogen fermentation microflora, we tested heat stress (105°C, 2 h) and freeze-thaw stress (a total of 3 cycles, each including-15°C, 12 h and 4°C, 6 h). The effect of bacterial stress on glucose hydrogen fermentation characteristic was determined by batch test using glass bottles. The results of the batch cultivation indicated that hydrogen fermentation characteristics differ among the stressed microflora. This result suggests that each original seed source has the suitable bacterial stress treatment to increase the hydrogen production. At the same time, we conducted 16S rRNA gene based analysis of hydrogen fermentation microbial community by PCR-minigel-single-strand conformation polymorphism (PCR-minigel-SSCP) method. Clostridium species such as Clostridium butyricum were identified from hydrogen fermentation batch culture.

Original languageEnglish
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008, ASABE 2008
PublisherAmerican Society of Agricultural and Biological Engineers
Pages3201-3211
Number of pages11
ISBN (Print)9781605605364
Publication statusPublished - 2008 Jan 1
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008 - Providence, RI, United States
Duration: 2008 Jun 292008 Jul 2

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008, ASABE 2008
Volume5

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008
CountryUnited States
CityProvidence, RI
Period08/6/2908/7/2

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Keywords

  • Bacterial stress
  • Freeze-thaw stress
  • Heat stress
  • Hydrogen fermentation
  • Microbial community
  • PCR-SSCP

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Kashima, H., Nyunoya, H., Oto, M., & Tojo, S. (2008). Effect of bacterial stress on hydrogen fermentation microflora. In American Society of Agricultural and Biological Engineers Annual International Meeting 2008, ASABE 2008 (pp. 3201-3211). (American Society of Agricultural and Biological Engineers Annual International Meeting 2008, ASABE 2008; Vol. 5). American Society of Agricultural and Biological Engineers.