Effect of Chloride Ion on the Thermal Decay Process of the Batho Intermediate of Iodopsin at Low Temperature

Yasushi Imamoto, Hideki Kandori, Toshiyuki Okano, Yoshitaka Fukada, Yoshinori Shichida, Tôru Yoshizawa*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

The photochemical and the subsequent thermal behaviors or iodopsin (Cl--bound form) and N-iodopsin (iodopsin whose Cl- was replaced by NO3-) in CHAPS-phosphatidylcholine (PC) were studied by low-temperature spectrophotometry. Irradiation of the iodopsin preparation at -185 °C produced a photo-steady-state mixture composed of iodopsin, bathoiodopsin, and isoiodopsin. Bathoiodopsin was thermally reverted to the original iodopsin. These results were almost the same as those reported previously [Yoshizawa, T., & Wald, G.(1967) Nature 214, 566-571] in which iodopsin was extracted with 2% digitonin. Therefore, photochemical and subsequent thermal behaviors or iodopsin were independent of the detergent to solubilize iodopsin. Irradiation of N-iodopsin at -185 °C produced the similar photo-steady-state mixture. However, N-bathoiodopsi was thermally converted to the next intermediate, presumably N-lumiiodopsin. These results suggest that the batho-lumi transition of iodopsin at low temperature is likely to be inhibited by the Cl- bound to the protein moiety of iodopsin, while at room temperature the Cl- bound to iodopsin could be released on the conversion process of batho- to lumiiodopsin.

Original languageEnglish
Pages (from-to)9412-9416
Number of pages5
JournalBiochemistry
Volume28
Issue number24
DOIs
Publication statusPublished - 1989 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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