Effect of chronic ethanol exposure on the liver of Clock-mutant mice

Takashi Kudo, Toru Tamagawa, Shigenobu Shibata

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    In humans, chronic ethanol consumption leads to a characteristic set of changes to the metabolism of lipids in the liver that is referred to as an "alcoholic fatty liver (AFL)". In severe cases, these metabolic changes result in the enlargement and fibrillization of the liver and are considered risk factors for cirrhosis and liver cancer. Clock-mutant mice have been shown to display abnormal lipid metabolism and alcohol preferences. To further understand the potential interactions between ethanol consumption, lipid metabolism, and the circadian clock, we investigated the effect of chronic ethanol intake on the lipid metabolism of Clock-mutant mice. We found that ethanol treatment produced a number of changes in the liver of Clock-mutant mice without impacting the wild-type controls. First, we found that 8 weeks of exposure to ethanol in the drinking water increased the weight of the liver in Clock-mutant mice. Ethanol treatment also increased triglyceride content of liver in Clock-mutant and wild-type mice. This increase was larger in the mutant mice. Finally, ethanol treatment altered the expression of a number of genes related to lipid metabolism in the Clock-mutant mice. Interestingly, this treatment did not impact circadian clock gene expression in the liver of either genotype. Thus, ethanol produces a number of changes in the liver of Clock-mutant mice that are not seen in the wild-type mice. These changes are consistent with the possibility that disturbance of circadian rhythmicity associated with the Clock mutation could be a risk factor for the development of an alcoholic fatty liver.

    Original languageEnglish
    Article number14
    JournalJournal of Circadian Rhythms
    Volume7
    DOIs
    Publication statusPublished - 2009 Apr 1

    Fingerprint

    Ethanol
    Liver
    Lipid Metabolism
    Alcoholic Fatty Liver
    Circadian Clocks
    Periodicity
    Liver Neoplasms
    Drinking Water
    Triglycerides
    Fibrosis
    Genotype
    Alcohols
    Gene Expression
    Weights and Measures
    Mutation
    Genes

    ASJC Scopus subject areas

    • Physiology
    • Endocrine and Autonomic Systems

    Cite this

    Effect of chronic ethanol exposure on the liver of Clock-mutant mice. / Kudo, Takashi; Tamagawa, Toru; Shibata, Shigenobu.

    In: Journal of Circadian Rhythms, Vol. 7, 14, 01.04.2009.

    Research output: Contribution to journalArticle

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