Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils

Qiang Liu, Katsuhiko Suzuki, Satoru Kudo, Mutsuo Yamada, Kenji Kowatari, Takashi Umeda, Shigeyuki Nakaji, Kazuo Sugawara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced chemiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils.

Original languageEnglish
Pages (from-to)327-330
Number of pages4
JournalLuminescence
Volume14
Issue number6
Publication statusPublished - 1999 Nov
Externally publishedYes

Fingerprint

Chemiluminescence
Luminescence
Neutrophils
Zymosan
Reactive Oxygen Species
Acetates
Food additives
Luminol
Food Additives
decaglycerol monooleate
Light absorption
Assays
Esters
Fatty Acids
Down-Regulation

Keywords

  • Chemiluminescence
  • Decaglycerol mono-oleate
  • Human neutrophil
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biophysics

Cite this

Liu, Q., Suzuki, K., Kudo, S., Yamada, M., Kowatari, K., Umeda, T., ... Sugawara, K. (1999). Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils. Luminescence, 14(6), 327-330.

Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils. / Liu, Qiang; Suzuki, Katsuhiko; Kudo, Satoru; Yamada, Mutsuo; Kowatari, Kenji; Umeda, Takashi; Nakaji, Shigeyuki; Sugawara, Kazuo.

In: Luminescence, Vol. 14, No. 6, 11.1999, p. 327-330.

Research output: Contribution to journalArticle

Liu, Q, Suzuki, K, Kudo, S, Yamada, M, Kowatari, K, Umeda, T, Nakaji, S & Sugawara, K 1999, 'Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils', Luminescence, vol. 14, no. 6, pp. 327-330.
Liu Q, Suzuki K, Kudo S, Yamada M, Kowatari K, Umeda T et al. Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils. Luminescence. 1999 Nov;14(6):327-330.
Liu, Qiang ; Suzuki, Katsuhiko ; Kudo, Satoru ; Yamada, Mutsuo ; Kowatari, Kenji ; Umeda, Takashi ; Nakaji, Shigeyuki ; Sugawara, Kazuo. / Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils. In: Luminescence. 1999 ; Vol. 14, No. 6. pp. 327-330.
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