Effect of extracellular matrix stiffness on ductular formation of biliary epithelial cells

Tomoya Komatsu, Ryo Sudo, Toshihiro Mitaka, Mariko Ikeda, Kazuo Tanishita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

There are increasing demands for the transplantation of tissue-engineered organs. An organ is composed of interconnected networks of ductular structures which transport vital fluids. It is therefore necessary to clarify the controlling factors of three dimensional ductular formation in vitro. Extracellular matrix (ECM) has been reported to be important for ductular formation. Recent studies showed that not only biological signals but also mechanical signals from ECM affected functions and morphology of cells. We hypothesized that the mechanical property of ECM was a controlling factor of three dimensional ductular formation as well. We previously reported that rat biliary epithelial cells (BECs), which constitute bile ducts in liver, reconstructed bile ducts in collagen gel sandwich culture with dimethylsulfoxide (DMSO) stimulation. Here, we investigated the effect of ECM stiffness on ductular formation of BECs. We found that ductular formation of BECs depended on collagen gel stiffness. As collagen gel became stiffer, BECs formed larger bile ducts. Real-time qPCR analysis also revealed that Ki-67 expression was independent of collagen gel stiffness and that Secretin reccptor (SR) and cystic fibrosis transmembrane conductance regulator (CFTR) mRNA expressions of large bile ducts reconstructed in the stiff collagen gel were higher than those of small bile ducts in the soft collagen gel. These data suggest that enlargement of the bile ducts by the stiff collagen gel is caused by migration of BECs rather than proliferation and that BEC functions depend on the size of reconstructed bile ducts as seen in vivo. In conclusion, we demonstrated that collagen gel stiffness affected the size of the reconstructed bile ducts, which is also leading to the different functions of the bile ducts depending on the size.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages1095-1098
Number of pages4
Volume31 IFMBE
DOIs
Publication statusPublished - 2010
Externally publishedYes
Event6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore
Duration: 2010 Aug 12010 Aug 6

Other

Other6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
CitySingapore
Period10/8/110/8/6

Fingerprint

Stiffness matrix
Ducts
Collagen
Gels
Stiffness
Epithelial Cells
Cystic Fibrosis Transmembrane Conductance Regulator
Secretin
Dimethyl Sulfoxide
Liver
Rats
Tissue
Mechanical properties
Messenger RNA
Fluids

Keywords

  • biliary epithelial cells
  • duct formation
  • elasticity
  • Extracellular matrix

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Komatsu, T., Sudo, R., Mitaka, T., Ikeda, M., & Tanishita, K. (2010). Effect of extracellular matrix stiffness on ductular formation of biliary epithelial cells. In IFMBE Proceedings (Vol. 31 IFMBE, pp. 1095-1098) https://doi.org/10.1007/978-3-642-14515-5_278

Effect of extracellular matrix stiffness on ductular formation of biliary epithelial cells. / Komatsu, Tomoya; Sudo, Ryo; Mitaka, Toshihiro; Ikeda, Mariko; Tanishita, Kazuo.

IFMBE Proceedings. Vol. 31 IFMBE 2010. p. 1095-1098.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Komatsu, T, Sudo, R, Mitaka, T, Ikeda, M & Tanishita, K 2010, Effect of extracellular matrix stiffness on ductular formation of biliary epithelial cells. in IFMBE Proceedings. vol. 31 IFMBE, pp. 1095-1098, 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech, Singapore, 10/8/1. https://doi.org/10.1007/978-3-642-14515-5_278
Komatsu T, Sudo R, Mitaka T, Ikeda M, Tanishita K. Effect of extracellular matrix stiffness on ductular formation of biliary epithelial cells. In IFMBE Proceedings. Vol. 31 IFMBE. 2010. p. 1095-1098 https://doi.org/10.1007/978-3-642-14515-5_278
Komatsu, Tomoya ; Sudo, Ryo ; Mitaka, Toshihiro ; Ikeda, Mariko ; Tanishita, Kazuo. / Effect of extracellular matrix stiffness on ductular formation of biliary epithelial cells. IFMBE Proceedings. Vol. 31 IFMBE 2010. pp. 1095-1098
@inproceedings{3199ee1a7fb84d90838d34abcebb761e,
title = "Effect of extracellular matrix stiffness on ductular formation of biliary epithelial cells",
abstract = "There are increasing demands for the transplantation of tissue-engineered organs. An organ is composed of interconnected networks of ductular structures which transport vital fluids. It is therefore necessary to clarify the controlling factors of three dimensional ductular formation in vitro. Extracellular matrix (ECM) has been reported to be important for ductular formation. Recent studies showed that not only biological signals but also mechanical signals from ECM affected functions and morphology of cells. We hypothesized that the mechanical property of ECM was a controlling factor of three dimensional ductular formation as well. We previously reported that rat biliary epithelial cells (BECs), which constitute bile ducts in liver, reconstructed bile ducts in collagen gel sandwich culture with dimethylsulfoxide (DMSO) stimulation. Here, we investigated the effect of ECM stiffness on ductular formation of BECs. We found that ductular formation of BECs depended on collagen gel stiffness. As collagen gel became stiffer, BECs formed larger bile ducts. Real-time qPCR analysis also revealed that Ki-67 expression was independent of collagen gel stiffness and that Secretin reccptor (SR) and cystic fibrosis transmembrane conductance regulator (CFTR) mRNA expressions of large bile ducts reconstructed in the stiff collagen gel were higher than those of small bile ducts in the soft collagen gel. These data suggest that enlargement of the bile ducts by the stiff collagen gel is caused by migration of BECs rather than proliferation and that BEC functions depend on the size of reconstructed bile ducts as seen in vivo. In conclusion, we demonstrated that collagen gel stiffness affected the size of the reconstructed bile ducts, which is also leading to the different functions of the bile ducts depending on the size.",
keywords = "biliary epithelial cells, duct formation, elasticity, Extracellular matrix",
author = "Tomoya Komatsu and Ryo Sudo and Toshihiro Mitaka and Mariko Ikeda and Kazuo Tanishita",
year = "2010",
doi = "10.1007/978-3-642-14515-5_278",
language = "English",
isbn = "9783540790389",
volume = "31 IFMBE",
pages = "1095--1098",
booktitle = "IFMBE Proceedings",

}

TY - GEN

T1 - Effect of extracellular matrix stiffness on ductular formation of biliary epithelial cells

AU - Komatsu, Tomoya

AU - Sudo, Ryo

AU - Mitaka, Toshihiro

AU - Ikeda, Mariko

AU - Tanishita, Kazuo

PY - 2010

Y1 - 2010

N2 - There are increasing demands for the transplantation of tissue-engineered organs. An organ is composed of interconnected networks of ductular structures which transport vital fluids. It is therefore necessary to clarify the controlling factors of three dimensional ductular formation in vitro. Extracellular matrix (ECM) has been reported to be important for ductular formation. Recent studies showed that not only biological signals but also mechanical signals from ECM affected functions and morphology of cells. We hypothesized that the mechanical property of ECM was a controlling factor of three dimensional ductular formation as well. We previously reported that rat biliary epithelial cells (BECs), which constitute bile ducts in liver, reconstructed bile ducts in collagen gel sandwich culture with dimethylsulfoxide (DMSO) stimulation. Here, we investigated the effect of ECM stiffness on ductular formation of BECs. We found that ductular formation of BECs depended on collagen gel stiffness. As collagen gel became stiffer, BECs formed larger bile ducts. Real-time qPCR analysis also revealed that Ki-67 expression was independent of collagen gel stiffness and that Secretin reccptor (SR) and cystic fibrosis transmembrane conductance regulator (CFTR) mRNA expressions of large bile ducts reconstructed in the stiff collagen gel were higher than those of small bile ducts in the soft collagen gel. These data suggest that enlargement of the bile ducts by the stiff collagen gel is caused by migration of BECs rather than proliferation and that BEC functions depend on the size of reconstructed bile ducts as seen in vivo. In conclusion, we demonstrated that collagen gel stiffness affected the size of the reconstructed bile ducts, which is also leading to the different functions of the bile ducts depending on the size.

AB - There are increasing demands for the transplantation of tissue-engineered organs. An organ is composed of interconnected networks of ductular structures which transport vital fluids. It is therefore necessary to clarify the controlling factors of three dimensional ductular formation in vitro. Extracellular matrix (ECM) has been reported to be important for ductular formation. Recent studies showed that not only biological signals but also mechanical signals from ECM affected functions and morphology of cells. We hypothesized that the mechanical property of ECM was a controlling factor of three dimensional ductular formation as well. We previously reported that rat biliary epithelial cells (BECs), which constitute bile ducts in liver, reconstructed bile ducts in collagen gel sandwich culture with dimethylsulfoxide (DMSO) stimulation. Here, we investigated the effect of ECM stiffness on ductular formation of BECs. We found that ductular formation of BECs depended on collagen gel stiffness. As collagen gel became stiffer, BECs formed larger bile ducts. Real-time qPCR analysis also revealed that Ki-67 expression was independent of collagen gel stiffness and that Secretin reccptor (SR) and cystic fibrosis transmembrane conductance regulator (CFTR) mRNA expressions of large bile ducts reconstructed in the stiff collagen gel were higher than those of small bile ducts in the soft collagen gel. These data suggest that enlargement of the bile ducts by the stiff collagen gel is caused by migration of BECs rather than proliferation and that BEC functions depend on the size of reconstructed bile ducts as seen in vivo. In conclusion, we demonstrated that collagen gel stiffness affected the size of the reconstructed bile ducts, which is also leading to the different functions of the bile ducts depending on the size.

KW - biliary epithelial cells

KW - duct formation

KW - elasticity

KW - Extracellular matrix

UR - http://www.scopus.com/inward/record.url?scp=77958016658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958016658&partnerID=8YFLogxK

U2 - 10.1007/978-3-642-14515-5_278

DO - 10.1007/978-3-642-14515-5_278

M3 - Conference contribution

AN - SCOPUS:77958016658

SN - 9783540790389

VL - 31 IFMBE

SP - 1095

EP - 1098

BT - IFMBE Proceedings

ER -