Effect of fluid force on vascular cell function

Susumu Kudo, Ryuhei Yamaguchi, Mariko Ikeda, Kazuo Tanishita

Research output: Contribution to journalArticle

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Abstract

Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to fluid frictional force (shear stress) induced by blood flow, and shear stress affects the intracellular calcium ([Ca 2+] i), which initiates cellular responses. Here, we studied the effect of long-term exposure of shear stress on [Ca 2+] i responses in cultured ECs by using a confocal laser microscope and calcium indicator. At the initiation of shear stress of 20dyn/cm 2 (0 hr), 27% of the cells exhibited [Ca 2+] i responses. This percentage gradually decreased with increasing exposure time, reaching about 4% after 24 hr of exposure. These data indicate that long-term shear-stress exposure affects [Ca 2+] i responses in cultured ECs. Furthermore, we studied the effect of magnitude of shear stress on macromolecule uptake. For the low shear-stress, the uptake was enhanced, whereas the uptake was inhibited for higher shear-stress.

Original languageEnglish
Pages (from-to)459-461
Number of pages3
JournalJournal of Physiological Anthropology and Applied Human Science
Volume24
Issue number4
DOIs
Publication statusPublished - 2005 Jul
Externally publishedYes

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Keywords

  • Bio-fluid mechanics
  • Calciumion
  • Endothelial cells
  • Shear flow

ASJC Scopus subject areas

  • Social Sciences(all)

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