Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum

Shi Shu; Taro Q.P. Uyeda; Aixiao Liu; Guoqin Liu; Longfei Yen

doi:10.1007/BF02885026

Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum

Shi Shu, Taro Q.P. Uyeda, Aixiao Liu, Guoqin Liu, Longfei Yen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Four deletion mutant Dictyostelium myosin II heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin II heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin IIs under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin II affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.

Original language	English
Pages (from-to)	811-816
Number of pages	6
Journal	Chinese Science Bulletin
Volume	44
Issue number	9
DOIs	https://doi.org/10.1007/BF02885026
Publication status	Published - 1999 May
Externally published	Yes

Keywords

Deletion mutation
Dictyostelium
Growth and development
Myosin II

ASJC Scopus subject areas

General

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10.1007/BF02885026

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@article{3755ba1b22f6472396ac8714a717510e,

title = "Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum",

abstract = "Four deletion mutant Dictyostelium myosin II heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin II heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin IIs under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin II affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.",

keywords = "Deletion mutation, Dictyostelium, Growth and development, Myosin II",

author = "Shi Shu and Uyeda, {Taro Q.P.} and Aixiao Liu and Guoqin Liu and Longfei Yen",

note = "Funding Information: Acknowledgement The authors thank Profs. Yang Shijie and Yu Bingsheng for their valuable suggestions. This work was camied out in National Institute for Advanced Interdisciplinary Research, Agency for Industrial Science and Technology, Ministry of International Trade and Industry, Japan. It was supported in part by the National Natural Science Foundation of China (Grant No. 39670354) .",

year = "1999",

month = may,

doi = "10.1007/BF02885026",

language = "English",

volume = "44",

pages = "811--816",

journal = "Science Bulletin",

issn = "2095-9273",

publisher = "Springer Science + Business Media",

number = "9",

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TY - JOUR

T1 - Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum

AU - Shu, Shi

AU - Uyeda, Taro Q.P.

AU - Liu, Aixiao

AU - Liu, Guoqin

AU - Yen, Longfei

N1 - Funding Information: Acknowledgement The authors thank Profs. Yang Shijie and Yu Bingsheng for their valuable suggestions. This work was camied out in National Institute for Advanced Interdisciplinary Research, Agency for Industrial Science and Technology, Ministry of International Trade and Industry, Japan. It was supported in part by the National Natural Science Foundation of China (Grant No. 39670354) .

PY - 1999/5

Y1 - 1999/5

N2 - Four deletion mutant Dictyostelium myosin II heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin II heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin IIs under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin II affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.

AB - Four deletion mutant Dictyostelium myosin II heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin II heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin IIs under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin II affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.

KW - Deletion mutation

KW - Dictyostelium

KW - Growth and development

KW - Myosin II

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JO - Science Bulletin

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Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum

Abstract

Keywords

ASJC Scopus subject areas

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