Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum

Shi Shu, Taro Q.P. Uyeda, Aixiao Liu, Guoqin Liu, Longfei Yen

Research output: Contribution to journalArticle

Abstract

Four deletion mutant Dictyostelium myosin II heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin II heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin IIs under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin II affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.

Original languageEnglish
Pages (from-to)811-816
Number of pages6
JournalChinese Science Bulletin
Volume44
Issue number9
DOIs
Publication statusPublished - 1999 May
Externally publishedYes

Keywords

  • Deletion mutation
  • Dictyostelium
  • Growth and development
  • Myosin II

ASJC Scopus subject areas

  • General

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