TY - JOUR
T1 - Effect of leptin on hypothalamic gene expression in calorie-restricted rats
AU - Komatsu, Toshimitsu
AU - Chiba, Takuya
AU - Yamaza, Haruyoshi
AU - To, Kazuo
AU - Toyama, Hiroaki
AU - Higami, Yoshikazu
AU - Shimokawa, Isao
N1 - Funding Information:
ACKNOWLEDGMENTS This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No. 12680779 and 15390128).
PY - 2006/9
Y1 - 2006/9
N2 - Diminished leptin signaling to the arcuate nucleus of hypothalamus (ARH) may induce calorie restriction (CR)-specific neuroendocrine and metabolic adaptation, which is potentially relevant to the effect of CR. The present study investigated whether restoration of leptin signaling to the ARH could reverse CR-induced alterations in neuropeptide gene expression in rats. Male F344 rats, fed ad libitum or a 30% CR diet from 6 weeks of age, received leptin or vehicle intracerebroventricularly for 14 days via osmotic mini-pumps implanted in the subcutis at 34 weeks of age. The messenger RNA levels were quantified by real-time reverse transcription-polymerase chain reaction using total RNA extracted from microdissected tissues containing the ARH. The results indicated that leptin administration reversed the upregulated expression of neuropeptide Y and agouti-related protein genes in CR rats, suggesting the possibility of a role for the leptin-ARH pathway in the effect of CR.
AB - Diminished leptin signaling to the arcuate nucleus of hypothalamus (ARH) may induce calorie restriction (CR)-specific neuroendocrine and metabolic adaptation, which is potentially relevant to the effect of CR. The present study investigated whether restoration of leptin signaling to the ARH could reverse CR-induced alterations in neuropeptide gene expression in rats. Male F344 rats, fed ad libitum or a 30% CR diet from 6 weeks of age, received leptin or vehicle intracerebroventricularly for 14 days via osmotic mini-pumps implanted in the subcutis at 34 weeks of age. The messenger RNA levels were quantified by real-time reverse transcription-polymerase chain reaction using total RNA extracted from microdissected tissues containing the ARH. The results indicated that leptin administration reversed the upregulated expression of neuropeptide Y and agouti-related protein genes in CR rats, suggesting the possibility of a role for the leptin-ARH pathway in the effect of CR.
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U2 - 10.1093/gerona/61.9.890
DO - 10.1093/gerona/61.9.890
M3 - Article
C2 - 16960019
AN - SCOPUS:33748773641
SN - 1079-5006
VL - 61
SP - 890
EP - 898
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 9
ER -