Effect of linear polarized near-infrared ray irradiation on the chemiluminescence of human neutrophils and serum opsonic activity

Masahiko Shiraishi, Katsuhiko Suzuki, Shigeyuki Nakaji, Kazuo Sugawara, Naoto Sugita, Koh Jun Suzuki, Seikou Ohta

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The purpose of this study was to investigate the in vitro effects of linear polarized near-infrared ray irradiation on neutrophil chemiluminescence (CL) and serum opsonic activity. We used luminol- and lucigenin-dependent CL to detect the affected reactive oxygen species production process of human neutrophils and measured serum opsonic activity based on luminol-dependent CL. The linear polarized near-infrared ray irradiation suppressed a maximum light emission (peak height) of luminol- and lucigenin-dependent CL in a dose-dependent manner. The findings suggested that the linear polarized near-infrared ray irradiation suppressed the superoxide anion and hypochlorite production of human neutrophils. The serum opsonic activity was decreased by linear polarized near-infrared ray irradiation, and this suppressive effect might be caused by inhibiting the activation of the classical and alternative complement pathway. Therefore, it is suggested that near-infrared ray irradiation may have an inhibitory effect against chronic pain via reduction of reactive oxygen species production and opsonic activity.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalLuminescence
Volume14
Issue number5
DOIs
Publication statusPublished - 1999 Jan 1

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Keywords

  • Human neutrophils
  • Linear polarized near-infrared ray
  • Reactive oxygen species
  • Serum opsonic activity

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

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