Abstract
The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.
| Original language | English |
|---|---|
| Pages (from-to) | 387-389 |
| Number of pages | 3 |
| Journal | Materials Science and Engineering C |
| Volume | 24 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2004 Apr 1 |
| Externally published | Yes |
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Keywords
- C2C12 myogenic cells
- Mechanical stretch
- TGF-β1 expression
ASJC Scopus subject areas
- Biomaterials
Cite this
Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells. / Akimoto, Takayuki; Ushida, Takashi; Miyaki, Shigeru; Akaogi, Hiroshi; Tateishi, Tetsuya; Fukubayashi, Toru.
In: Materials Science and Engineering C, Vol. 24, No. 3, 01.04.2004, p. 387-389.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells
AU - Akimoto, Takayuki
AU - Ushida, Takashi
AU - Miyaki, Shigeru
AU - Akaogi, Hiroshi
AU - Tateishi, Tetsuya
AU - Fukubayashi, Toru
PY - 2004/4/1
Y1 - 2004/4/1
N2 - The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.
AB - The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.
KW - C2C12 myogenic cells
KW - Mechanical stretch
KW - TGF-β1 expression
UR - http://www.scopus.com/inward/record.url?scp=1842679410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1842679410&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2003.12.013
DO - 10.1016/j.msec.2003.12.013
M3 - Article
VL - 24
SP - 387
EP - 389
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
IS - 3
ER -