Abstract
The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.
Original language | English |
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Pages (from-to) | 387-389 |
Number of pages | 3 |
Journal | Materials Science and Engineering C |
Volume | 24 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2004 Apr 1 |
Externally published | Yes |
Keywords
- C2C12 myogenic cells
- Mechanical stretch
- TGF-β1 expression
ASJC Scopus subject areas
- Materials Science(all)
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering