Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

Takayuki Akimoto; Takashi Ushida; Shigeru Miyaki; Hiroshi Akaogi; Tetsuya Tateishi; Toru Fukubayashi

doi:10.1016/j.msec.2003.12.013

Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

Takayuki Akimoto, Takashi Ushida, Shigeru Miyaki, Hiroshi Akaogi, Tetsuya Tateishi, Toru Fukubayashi

School of Sport Sciences

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.

Original language	English
Pages (from-to)	387-389
Number of pages	3
Journal	Materials Science and Engineering C
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/j.msec.2003.12.013
Publication status	Published - 2004 Apr 1

Keywords

C2C12 myogenic cells
Mechanical stretch
TGF-β1 expression

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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Akimoto, T., Ushida, T., Miyaki, S., Akaogi, H., Tateishi, T., & Fukubayashi, T. (2004). Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells. Materials Science and Engineering C, 24(3), 387-389. https://doi.org/10.1016/j.msec.2003.12.013

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abstract = "The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.",

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AU - Miyaki, Shigeru

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AU - Tateishi, Tetsuya

AU - Fukubayashi, Toru

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