Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

Takayuki Akimoto*, Takashi Ushida, Shigeru Miyaki, Hiroshi Akaogi, Tetsuya Tateishi, Toru Fukubayashi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.

Original languageEnglish
Pages (from-to)387-389
Number of pages3
JournalMaterials Science and Engineering C
Issue number3
Publication statusPublished - 2004 Apr 1
Externally publishedYes


  • C2C12 myogenic cells
  • Mechanical stretch
  • TGF-β1 expression

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering


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