Effect of monoamines on field potentials in the suprachiasmatic nucleus of slices of hypothalamus of the rat evoked by stimulation of the optic nerve

S. Y. Liou, Shigenobu Shibata, S. Ueki

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41 Citations (Scopus)

Abstract

The effects of the application of serotonin, histamine, noradrenaline and dopamine to the bath on field potentials in the suprachiasmatic nucleus evoked by stimulation of the optic nerve were studied using a hypothalamic slice. Stimulation of the contralateral optic nerve evoked fast positive and late large negative waves in the suprachiasmatic nucleus. The monoamines produced a dose dependent suppression of the amplitude of the negative wave but did not affect that of the positive waves, and the order of potency was serotonin > noradrenaline > dopamine ≥ histamine. The negative wave was suppressed by phenylephrine (0.1-10 μ M) in a dose-dependent manner, whereas it was unaffected by isoproterenol (0.1-10 μM). The suppression of the negative wave produced by the application of histamine and noradrenaline was antagonized by the H1-receptor antagonist, diphenhydramine and the α1-receptor antagonists, phenoxybenzamine and phentolamine. Therefore, the suppression of the negative wave by histamine and noradrenaline was mediated by the H1-receptor and α1-receptor, respectively. The present study indicates that monoamines may play an inhibitory role in the regulation of neurotransmission in the retinohypothalamic pathway to the suprachiasmatic nucleus.

Original languageEnglish
Pages (from-to)1009-1014
Number of pages6
JournalNeuropharmacology
Volume25
Issue number9
DOIs
Publication statusPublished - 1986
Externally publishedYes

Fingerprint

Suprachiasmatic Nucleus
Optic Nerve
Histamine
Hypothalamus
Norepinephrine
Histamine H1 Receptors
Dopamine
Serotonin
Diphenhydramine
Phenoxybenzamine
Phentolamine
Phenylephrine
Baths
Isoproterenol
Synaptic Transmission

Keywords

  • field potential
  • monoamines
  • retionohypothalamic pathway
  • Suprachiasmatic nucleus
  • tissue slice

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

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title = "Effect of monoamines on field potentials in the suprachiasmatic nucleus of slices of hypothalamus of the rat evoked by stimulation of the optic nerve",
abstract = "The effects of the application of serotonin, histamine, noradrenaline and dopamine to the bath on field potentials in the suprachiasmatic nucleus evoked by stimulation of the optic nerve were studied using a hypothalamic slice. Stimulation of the contralateral optic nerve evoked fast positive and late large negative waves in the suprachiasmatic nucleus. The monoamines produced a dose dependent suppression of the amplitude of the negative wave but did not affect that of the positive waves, and the order of potency was serotonin > noradrenaline > dopamine ≥ histamine. The negative wave was suppressed by phenylephrine (0.1-10 μ M) in a dose-dependent manner, whereas it was unaffected by isoproterenol (0.1-10 μM). The suppression of the negative wave produced by the application of histamine and noradrenaline was antagonized by the H1-receptor antagonist, diphenhydramine and the α1-receptor antagonists, phenoxybenzamine and phentolamine. Therefore, the suppression of the negative wave by histamine and noradrenaline was mediated by the H1-receptor and α1-receptor, respectively. The present study indicates that monoamines may play an inhibitory role in the regulation of neurotransmission in the retinohypothalamic pathway to the suprachiasmatic nucleus.",
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T1 - Effect of monoamines on field potentials in the suprachiasmatic nucleus of slices of hypothalamus of the rat evoked by stimulation of the optic nerve

AU - Liou, S. Y.

AU - Shibata, Shigenobu

AU - Ueki, S.

PY - 1986

Y1 - 1986

N2 - The effects of the application of serotonin, histamine, noradrenaline and dopamine to the bath on field potentials in the suprachiasmatic nucleus evoked by stimulation of the optic nerve were studied using a hypothalamic slice. Stimulation of the contralateral optic nerve evoked fast positive and late large negative waves in the suprachiasmatic nucleus. The monoamines produced a dose dependent suppression of the amplitude of the negative wave but did not affect that of the positive waves, and the order of potency was serotonin > noradrenaline > dopamine ≥ histamine. The negative wave was suppressed by phenylephrine (0.1-10 μ M) in a dose-dependent manner, whereas it was unaffected by isoproterenol (0.1-10 μM). The suppression of the negative wave produced by the application of histamine and noradrenaline was antagonized by the H1-receptor antagonist, diphenhydramine and the α1-receptor antagonists, phenoxybenzamine and phentolamine. Therefore, the suppression of the negative wave by histamine and noradrenaline was mediated by the H1-receptor and α1-receptor, respectively. The present study indicates that monoamines may play an inhibitory role in the regulation of neurotransmission in the retinohypothalamic pathway to the suprachiasmatic nucleus.

AB - The effects of the application of serotonin, histamine, noradrenaline and dopamine to the bath on field potentials in the suprachiasmatic nucleus evoked by stimulation of the optic nerve were studied using a hypothalamic slice. Stimulation of the contralateral optic nerve evoked fast positive and late large negative waves in the suprachiasmatic nucleus. The monoamines produced a dose dependent suppression of the amplitude of the negative wave but did not affect that of the positive waves, and the order of potency was serotonin > noradrenaline > dopamine ≥ histamine. The negative wave was suppressed by phenylephrine (0.1-10 μ M) in a dose-dependent manner, whereas it was unaffected by isoproterenol (0.1-10 μM). The suppression of the negative wave produced by the application of histamine and noradrenaline was antagonized by the H1-receptor antagonist, diphenhydramine and the α1-receptor antagonists, phenoxybenzamine and phentolamine. Therefore, the suppression of the negative wave by histamine and noradrenaline was mediated by the H1-receptor and α1-receptor, respectively. The present study indicates that monoamines may play an inhibitory role in the regulation of neurotransmission in the retinohypothalamic pathway to the suprachiasmatic nucleus.

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KW - Suprachiasmatic nucleus

KW - tissue slice

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