Effect of polyethylene glycol modification of TiO 2 nanoparticles on cytotoxicity and gene expressions in human cell lines

Sharmy Saimon Mano, Koki Kanehira, Shuji Sonezaki, Akiyoshi Taniguchi

    Research output: Contribution to journalArticle

    49 Citations (Scopus)

    Abstract

    Nanoparticles (NPs) are tiny materials used in a wide range of industrial and medical applications. Titanium dioxide (TiO 2) is a type of nanoparticle that is widely used in paints, pigments, and cosmetics; however, little is known about the impact of TiO 2 on human health and the environment. Therefore, considerable research has focused on characterizing the potential toxicity of nanoparticles such as TiO 2 and on understanding the mechanism of TiO 2 NP-induced nanotoxicity through the evaluation of biomarkers. Uncoated TiO 2 NPs tend to aggregate in aqueous media, and these aggregates decrease cell viability and induce expression of stress-related genes, such as those encoding interleukin-6 (IL-6) and heat shock protein 70B' (HSP70B'), indicating that TiO 2 NPs induce inflammatory and heat shock responses. In order to reduce their toxicity, we conjugated TiO 2 NPs with polyethylene glycol (PEG) to eliminate aggregation. Our findings indicate that modifying TiO 2 NPs with PEG reduces their cytotoxicity and reduces the induction of stress-related genes. Our results also suggest that TiO 2 NP-induced effects on cytotoxicity and gene expression vary depending upon the cell type and surface modification.

    Original languageEnglish
    Pages (from-to)3703-3737
    Number of pages35
    JournalInternational Journal of Molecular Sciences
    Volume13
    Issue number3
    DOIs
    Publication statusPublished - 2012 Mar

    Fingerprint

    gene expression
    Cytotoxicity
    cultured cells
    Gene expression
    Nanoparticles
    Polyethylene glycols
    glycols
    polyethylenes
    Cells
    Gene Expression
    Cell Line
    nanoparticles
    toxicity
    genes
    Toxicity
    Genes
    shock
    interleukins
    Heat-Shock Response
    heat

    Keywords

    • Gene expnressio
    • Nanoparticles
    • Nanotoxicology
    • Titanium dioxide

    ASJC Scopus subject areas

    • Computer Science Applications
    • Molecular Biology
    • Catalysis
    • Inorganic Chemistry
    • Spectroscopy
    • Organic Chemistry
    • Physical and Theoretical Chemistry

    Cite this

    Effect of polyethylene glycol modification of TiO 2 nanoparticles on cytotoxicity and gene expressions in human cell lines. / Mano, Sharmy Saimon; Kanehira, Koki; Sonezaki, Shuji; Taniguchi, Akiyoshi.

    In: International Journal of Molecular Sciences, Vol. 13, No. 3, 03.2012, p. 3703-3737.

    Research output: Contribution to journalArticle

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