Effect of shear stress on albumin uptake into cultured endothelial cells

Susumu Kudo, Kenji Ikezawa, Shinji Matsumura, Mariko Ikeda, Kotaro Oka, Kazuo Tanishita

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

The endothelial cell monolayer exposed to shear stress by a blood flow in vivo is a principal barrier for macromolecules transport across the vascular wall. Abnormal uptake of macromolecules into endothelial cell monolayer causes several vascular diseases. Therefore we studied the effect of shear stress on macromolecules uptake into cultured endothelial cells. After 48hr. exposure to shear stress. endothelial cells on coverslips were incubated at 37°C for 60 minutes in PBS containing Tetrametylrhodamine Isothiocyanate conjugated Albumin (TRITC-Albumin). Thereafter the uptake of albumin and the shape of endothelial cells were observed by a laser scanning confocal microscope. The uptake of albumin into endothelial cells exposed to shear stress increased for lower shear stress range and decreased for high shear stress.

Original languageEnglish
Title of host publicationAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Pages209-210
Number of pages2
Volume33
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Endothelial cells
Shear stress
Macromolecules
Monolayers
Microscopes
Blood
Scanning
Lasers

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kudo, S., Ikezawa, K., Matsumura, S., Ikeda, M., Oka, K., & Tanishita, K. (1996). Effect of shear stress on albumin uptake into cultured endothelial cells. In American Society of Mechanical Engineers, Bioengineering Division (Publication) BED (Vol. 33, pp. 209-210)

Effect of shear stress on albumin uptake into cultured endothelial cells. / Kudo, Susumu; Ikezawa, Kenji; Matsumura, Shinji; Ikeda, Mariko; Oka, Kotaro; Tanishita, Kazuo.

American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 33 1996. p. 209-210.

Research output: Chapter in Book/Report/Conference proceedingChapter

Kudo, S, Ikezawa, K, Matsumura, S, Ikeda, M, Oka, K & Tanishita, K 1996, Effect of shear stress on albumin uptake into cultured endothelial cells. in American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. vol. 33, pp. 209-210.
Kudo S, Ikezawa K, Matsumura S, Ikeda M, Oka K, Tanishita K. Effect of shear stress on albumin uptake into cultured endothelial cells. In American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 33. 1996. p. 209-210
Kudo, Susumu ; Ikezawa, Kenji ; Matsumura, Shinji ; Ikeda, Mariko ; Oka, Kotaro ; Tanishita, Kazuo. / Effect of shear stress on albumin uptake into cultured endothelial cells. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 33 1996. pp. 209-210
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