Effect of the size of receptor in allergy detection using field effect transistor biosensor

Sho Hideshima, Shigeki Kuroiwa, Marika Kimura, Shanshan Cheng, Tetsuya Osaka

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Allergy diagnosis, conducted to determine whether a specific syndrome is attributable to allergy, plays a significant role in the overall health examination. In this paper we report that the detection of allergy-associated protein, immunoglobulin E (IgE), was achieved by using field effect transistors (FETs) immobilized with an antigen as a receptor, which is smaller in size than the conventional receptor (antibody). The antigen-immobilized FETs exhibit a higher response to IgE than the antibody-immobilized FETs, suggesting that the smaller receptor not only makes the more effective use of the charge-detectable region for the FET-based detection in terms of Debye length, but also provides more recognition sites for target molecules and greater ability to block nonspecific adsorption of non-related proteins because of the closely-packed immobilized receptors. In addition, the application of the antigen to FET biosensor gives an advantage in the identification of the specific allergen. These results show that the small receptor of antigen is more effective than the antibody in the allergy detection using FET biosensors.

Original languageEnglish
Pages (from-to)146-151
Number of pages6
JournalElectrochimica Acta
Volume110
DOIs
Publication statusPublished - 2013 Aug 2

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Keywords

  • Allergy diagnosis
  • Field effect transistor biosensor
  • Immunoglobulin E
  • Ovalbumin

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

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