Effect of Zn and Mg in tricalcium phosphate and in culture medium on apoptosis and actin ring formation of mature osteoclasts

Xia Li, Koji Senda, Atsuo Ito, Yu Sogo, Atsushi Yamazaki

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    This study investigated the resorptive activity of osteoclasts on tricalcium phosphate (TCP), zinc-containing tricalcium phosphate (ZnTCP) and magnesium-containing tricalcium phosphate (MgTCP) ceramics in different Zn- or Mg-containing culture media. On the TCP ceramic, an increase in Zn ions in the culture medium within the range between 0.3 and 6.8 ppm significantly induced an increase in osteoclast apoptosis and a decrease in actin ring formation. However, even a high level of Mg ions up to 100 ppm in the culture medium was unlikely to induce an increase in osteoclast apoptosis. Mg ions in the MgTCP ceramics have no effect on osteoclast apoptosis and actin ring formation. There was almost no significant difference in osteoclast apoptosis and actin ring formation between ZnTCP and MgTCP ceramics which have the same solubility and dissolution rates. It is indicated that only an increase in Zn level outside resorption lacuna has an inhibitory effect on osteoclast resorption and that an increase in Zn level inside resorption lacuna could not influence the osteoclast activity.

    Original languageEnglish
    Article number045002
    JournalBiomedical Materials
    Volume3
    Issue number4
    DOIs
    Publication statusPublished - 2008 Dec 1

    Fingerprint

    Osteoclasts
    Cell death
    Cell culture
    Culture Media
    Actins
    Phosphates
    Apoptosis
    Magnesium
    Ions
    Zinc
    tricalcium phosphate
    Solubility
    Dissolution

    ASJC Scopus subject areas

    • Chemistry (miscellaneous)
    • Biochemistry
    • Mechanics of Materials
    • Medicine(all)

    Cite this

    Effect of Zn and Mg in tricalcium phosphate and in culture medium on apoptosis and actin ring formation of mature osteoclasts. / Li, Xia; Senda, Koji; Ito, Atsuo; Sogo, Yu; Yamazaki, Atsushi.

    In: Biomedical Materials, Vol. 3, No. 4, 045002, 01.12.2008.

    Research output: Contribution to journalArticle

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    AU - Senda, Koji

    AU - Ito, Atsuo

    AU - Sogo, Yu

    AU - Yamazaki, Atsushi

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    AB - This study investigated the resorptive activity of osteoclasts on tricalcium phosphate (TCP), zinc-containing tricalcium phosphate (ZnTCP) and magnesium-containing tricalcium phosphate (MgTCP) ceramics in different Zn- or Mg-containing culture media. On the TCP ceramic, an increase in Zn ions in the culture medium within the range between 0.3 and 6.8 ppm significantly induced an increase in osteoclast apoptosis and a decrease in actin ring formation. However, even a high level of Mg ions up to 100 ppm in the culture medium was unlikely to induce an increase in osteoclast apoptosis. Mg ions in the MgTCP ceramics have no effect on osteoclast apoptosis and actin ring formation. There was almost no significant difference in osteoclast apoptosis and actin ring formation between ZnTCP and MgTCP ceramics which have the same solubility and dissolution rates. It is indicated that only an increase in Zn level outside resorption lacuna has an inhibitory effect on osteoclast resorption and that an increase in Zn level inside resorption lacuna could not influence the osteoclast activity.

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