Abstract
Objective: To investigate the efficacy of multioverlapping therapy using a polysaccharide nanosheet having 75-nm thickness for sealing and stopping massive venous hemorrhage. Methods: The hydrostatic durability of the polysaccharide nanosheet was evaluated invitro when secured to an incised silicon tube. For invivo studies, the inferior vena cava (IVC) of rabbits was cut longitudinally, and multiple polysaccharide nanosheets were overlapped onto the injured IVC. Results: The mechanical hydrostatic durability of the nanosheets was gradually augmented by an increasing number of multilayered nanosheets invitro. This durability was saturated at 80± 6 mm Hg by four layers of nanosheets, which was robust enough to seal injured vessel walls of the large IVC. Multioverlapping therapy using nanosheets effectively sealed and stopped bleeding from the injured IVC invivo. One month later, no inflammatory tissue response was observed around the nanosheet attachment sites of the IVC, while conventional suturing repair in control rabbits showed a severe inflammatory response around the sutured area. Conclusions: The multioverlapping therapy using the polysaccharide nanosheets will effectively stop massive venous bleeding without adverse effects in the immediate or chronic postoperative setting. Clinical Relevance: Suturing repair is usually performed for perforated bleeding from proximal central veins such as the inferior vena cava; ligation of large veins is a limited option for damage control surgery. This study demonstrates the preliminary results of novel polysaccharide nanosheets for sutureless treatment to stop bleeding from the inferior vena cava. If these results are confirmed in humans with a significantly improved hemostasis maneuver, they would have a great advantage for surgical operation, especially in trauma patients with bleeding from large veins.
| Original language | English |
|---|---|
| Pages (from-to) | 289-297 |
| Number of pages | 9 |
| Journal | Journal of Vascular Surgery: Venous and Lymphatic Disorders |
| Volume | 1 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2013 Jul |
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ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Surgery
Cite this
Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model. / Hagisawa, Kohsuke; Saito, Akihiro; Kinoshita, Manabu; Fujie, Toshinori; Otani, Naoki; Shono, Satoshi; Park, Young Kwang; Takeoka, Shinji.
In: Journal of Vascular Surgery: Venous and Lymphatic Disorders, Vol. 1, No. 3, 07.2013, p. 289-297.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model
AU - Hagisawa, Kohsuke
AU - Saito, Akihiro
AU - Kinoshita, Manabu
AU - Fujie, Toshinori
AU - Otani, Naoki
AU - Shono, Satoshi
AU - Park, Young Kwang
AU - Takeoka, Shinji
PY - 2013/7
Y1 - 2013/7
N2 - Objective: To investigate the efficacy of multioverlapping therapy using a polysaccharide nanosheet having 75-nm thickness for sealing and stopping massive venous hemorrhage. Methods: The hydrostatic durability of the polysaccharide nanosheet was evaluated invitro when secured to an incised silicon tube. For invivo studies, the inferior vena cava (IVC) of rabbits was cut longitudinally, and multiple polysaccharide nanosheets were overlapped onto the injured IVC. Results: The mechanical hydrostatic durability of the nanosheets was gradually augmented by an increasing number of multilayered nanosheets invitro. This durability was saturated at 80± 6 mm Hg by four layers of nanosheets, which was robust enough to seal injured vessel walls of the large IVC. Multioverlapping therapy using nanosheets effectively sealed and stopped bleeding from the injured IVC invivo. One month later, no inflammatory tissue response was observed around the nanosheet attachment sites of the IVC, while conventional suturing repair in control rabbits showed a severe inflammatory response around the sutured area. Conclusions: The multioverlapping therapy using the polysaccharide nanosheets will effectively stop massive venous bleeding without adverse effects in the immediate or chronic postoperative setting. Clinical Relevance: Suturing repair is usually performed for perforated bleeding from proximal central veins such as the inferior vena cava; ligation of large veins is a limited option for damage control surgery. This study demonstrates the preliminary results of novel polysaccharide nanosheets for sutureless treatment to stop bleeding from the inferior vena cava. If these results are confirmed in humans with a significantly improved hemostasis maneuver, they would have a great advantage for surgical operation, especially in trauma patients with bleeding from large veins.
AB - Objective: To investigate the efficacy of multioverlapping therapy using a polysaccharide nanosheet having 75-nm thickness for sealing and stopping massive venous hemorrhage. Methods: The hydrostatic durability of the polysaccharide nanosheet was evaluated invitro when secured to an incised silicon tube. For invivo studies, the inferior vena cava (IVC) of rabbits was cut longitudinally, and multiple polysaccharide nanosheets were overlapped onto the injured IVC. Results: The mechanical hydrostatic durability of the nanosheets was gradually augmented by an increasing number of multilayered nanosheets invitro. This durability was saturated at 80± 6 mm Hg by four layers of nanosheets, which was robust enough to seal injured vessel walls of the large IVC. Multioverlapping therapy using nanosheets effectively sealed and stopped bleeding from the injured IVC invivo. One month later, no inflammatory tissue response was observed around the nanosheet attachment sites of the IVC, while conventional suturing repair in control rabbits showed a severe inflammatory response around the sutured area. Conclusions: The multioverlapping therapy using the polysaccharide nanosheets will effectively stop massive venous bleeding without adverse effects in the immediate or chronic postoperative setting. Clinical Relevance: Suturing repair is usually performed for perforated bleeding from proximal central veins such as the inferior vena cava; ligation of large veins is a limited option for damage control surgery. This study demonstrates the preliminary results of novel polysaccharide nanosheets for sutureless treatment to stop bleeding from the inferior vena cava. If these results are confirmed in humans with a significantly improved hemostasis maneuver, they would have a great advantage for surgical operation, especially in trauma patients with bleeding from large veins.
UR - http://www.scopus.com/inward/record.url?scp=84879239999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879239999&partnerID=8YFLogxK
U2 - 10.1016/j.jvsv.2013.04.001
DO - 10.1016/j.jvsv.2013.04.001
M3 - Article
AN - SCOPUS:84879239999
VL - 1
SP - 289
EP - 297
JO - Journal of Vascular Surgery: Venous and Lymphatic Disorders
JF - Journal of Vascular Surgery: Venous and Lymphatic Disorders
SN - 2213-333X
IS - 3
ER -