Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model

Kohsuke Hagisawa, Akihiro Saito, Manabu Kinoshita, Toshinori Fujie, Naoki Otani, Satoshi Shono, Young Kwang Park, Shinji Takeoka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To investigate the efficacy of multioverlapping therapy using a polysaccharide nanosheet having 75-nm thickness for sealing and stopping massive venous hemorrhage. Methods: The hydrostatic durability of the polysaccharide nanosheet was evaluated invitro when secured to an incised silicon tube. For invivo studies, the inferior vena cava (IVC) of rabbits was cut longitudinally, and multiple polysaccharide nanosheets were overlapped onto the injured IVC. Results: The mechanical hydrostatic durability of the nanosheets was gradually augmented by an increasing number of multilayered nanosheets invitro. This durability was saturated at 80± 6 mm Hg by four layers of nanosheets, which was robust enough to seal injured vessel walls of the large IVC. Multioverlapping therapy using nanosheets effectively sealed and stopped bleeding from the injured IVC invivo. One month later, no inflammatory tissue response was observed around the nanosheet attachment sites of the IVC, while conventional suturing repair in control rabbits showed a severe inflammatory response around the sutured area. Conclusions: The multioverlapping therapy using the polysaccharide nanosheets will effectively stop massive venous bleeding without adverse effects in the immediate or chronic postoperative setting. Clinical Relevance: Suturing repair is usually performed for perforated bleeding from proximal central veins such as the inferior vena cava; ligation of large veins is a limited option for damage control surgery. This study demonstrates the preliminary results of novel polysaccharide nanosheets for sutureless treatment to stop bleeding from the inferior vena cava. If these results are confirmed in humans with a significantly improved hemostasis maneuver, they would have a great advantage for surgical operation, especially in trauma patients with bleeding from large veins.

Original languageEnglish
Pages (from-to)289-297
Number of pages9
JournalJournal of Vascular Surgery: Venous and Lymphatic Disorders
Volume1
Issue number3
DOIs
Publication statusPublished - 2013 Jul

Fingerprint

Inferior Vena Cava
Polysaccharides
Hemorrhage
Rabbits
Wounds and Injuries
Veins
Therapeutics
Silicon
Hemostasis
Ligation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model. / Hagisawa, Kohsuke; Saito, Akihiro; Kinoshita, Manabu; Fujie, Toshinori; Otani, Naoki; Shono, Satoshi; Park, Young Kwang; Takeoka, Shinji.

In: Journal of Vascular Surgery: Venous and Lymphatic Disorders, Vol. 1, No. 3, 07.2013, p. 289-297.

Research output: Contribution to journalArticle

Hagisawa, Kohsuke ; Saito, Akihiro ; Kinoshita, Manabu ; Fujie, Toshinori ; Otani, Naoki ; Shono, Satoshi ; Park, Young Kwang ; Takeoka, Shinji. / Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model. In: Journal of Vascular Surgery: Venous and Lymphatic Disorders. 2013 ; Vol. 1, No. 3. pp. 289-297.
@article{338608f2d53e44478d896d76c1dee79a,
title = "Effective control of massive venous bleeding by {"}multioverlapping therapy{"} using polysaccharide nanosheets in a rabbit inferior vena cava injury model",
abstract = "Objective: To investigate the efficacy of multioverlapping therapy using a polysaccharide nanosheet having 75-nm thickness for sealing and stopping massive venous hemorrhage. Methods: The hydrostatic durability of the polysaccharide nanosheet was evaluated invitro when secured to an incised silicon tube. For invivo studies, the inferior vena cava (IVC) of rabbits was cut longitudinally, and multiple polysaccharide nanosheets were overlapped onto the injured IVC. Results: The mechanical hydrostatic durability of the nanosheets was gradually augmented by an increasing number of multilayered nanosheets invitro. This durability was saturated at 80± 6 mm Hg by four layers of nanosheets, which was robust enough to seal injured vessel walls of the large IVC. Multioverlapping therapy using nanosheets effectively sealed and stopped bleeding from the injured IVC invivo. One month later, no inflammatory tissue response was observed around the nanosheet attachment sites of the IVC, while conventional suturing repair in control rabbits showed a severe inflammatory response around the sutured area. Conclusions: The multioverlapping therapy using the polysaccharide nanosheets will effectively stop massive venous bleeding without adverse effects in the immediate or chronic postoperative setting. Clinical Relevance: Suturing repair is usually performed for perforated bleeding from proximal central veins such as the inferior vena cava; ligation of large veins is a limited option for damage control surgery. This study demonstrates the preliminary results of novel polysaccharide nanosheets for sutureless treatment to stop bleeding from the inferior vena cava. If these results are confirmed in humans with a significantly improved hemostasis maneuver, they would have a great advantage for surgical operation, especially in trauma patients with bleeding from large veins.",
author = "Kohsuke Hagisawa and Akihiro Saito and Manabu Kinoshita and Toshinori Fujie and Naoki Otani and Satoshi Shono and Park, {Young Kwang} and Shinji Takeoka",
year = "2013",
month = "7",
doi = "10.1016/j.jvsv.2013.04.001",
language = "English",
volume = "1",
pages = "289--297",
journal = "Journal of Vascular Surgery: Venous and Lymphatic Disorders",
issn = "2213-333X",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model

AU - Hagisawa, Kohsuke

AU - Saito, Akihiro

AU - Kinoshita, Manabu

AU - Fujie, Toshinori

AU - Otani, Naoki

AU - Shono, Satoshi

AU - Park, Young Kwang

AU - Takeoka, Shinji

PY - 2013/7

Y1 - 2013/7

N2 - Objective: To investigate the efficacy of multioverlapping therapy using a polysaccharide nanosheet having 75-nm thickness for sealing and stopping massive venous hemorrhage. Methods: The hydrostatic durability of the polysaccharide nanosheet was evaluated invitro when secured to an incised silicon tube. For invivo studies, the inferior vena cava (IVC) of rabbits was cut longitudinally, and multiple polysaccharide nanosheets were overlapped onto the injured IVC. Results: The mechanical hydrostatic durability of the nanosheets was gradually augmented by an increasing number of multilayered nanosheets invitro. This durability was saturated at 80± 6 mm Hg by four layers of nanosheets, which was robust enough to seal injured vessel walls of the large IVC. Multioverlapping therapy using nanosheets effectively sealed and stopped bleeding from the injured IVC invivo. One month later, no inflammatory tissue response was observed around the nanosheet attachment sites of the IVC, while conventional suturing repair in control rabbits showed a severe inflammatory response around the sutured area. Conclusions: The multioverlapping therapy using the polysaccharide nanosheets will effectively stop massive venous bleeding without adverse effects in the immediate or chronic postoperative setting. Clinical Relevance: Suturing repair is usually performed for perforated bleeding from proximal central veins such as the inferior vena cava; ligation of large veins is a limited option for damage control surgery. This study demonstrates the preliminary results of novel polysaccharide nanosheets for sutureless treatment to stop bleeding from the inferior vena cava. If these results are confirmed in humans with a significantly improved hemostasis maneuver, they would have a great advantage for surgical operation, especially in trauma patients with bleeding from large veins.

AB - Objective: To investigate the efficacy of multioverlapping therapy using a polysaccharide nanosheet having 75-nm thickness for sealing and stopping massive venous hemorrhage. Methods: The hydrostatic durability of the polysaccharide nanosheet was evaluated invitro when secured to an incised silicon tube. For invivo studies, the inferior vena cava (IVC) of rabbits was cut longitudinally, and multiple polysaccharide nanosheets were overlapped onto the injured IVC. Results: The mechanical hydrostatic durability of the nanosheets was gradually augmented by an increasing number of multilayered nanosheets invitro. This durability was saturated at 80± 6 mm Hg by four layers of nanosheets, which was robust enough to seal injured vessel walls of the large IVC. Multioverlapping therapy using nanosheets effectively sealed and stopped bleeding from the injured IVC invivo. One month later, no inflammatory tissue response was observed around the nanosheet attachment sites of the IVC, while conventional suturing repair in control rabbits showed a severe inflammatory response around the sutured area. Conclusions: The multioverlapping therapy using the polysaccharide nanosheets will effectively stop massive venous bleeding without adverse effects in the immediate or chronic postoperative setting. Clinical Relevance: Suturing repair is usually performed for perforated bleeding from proximal central veins such as the inferior vena cava; ligation of large veins is a limited option for damage control surgery. This study demonstrates the preliminary results of novel polysaccharide nanosheets for sutureless treatment to stop bleeding from the inferior vena cava. If these results are confirmed in humans with a significantly improved hemostasis maneuver, they would have a great advantage for surgical operation, especially in trauma patients with bleeding from large veins.

UR - http://www.scopus.com/inward/record.url?scp=84879239999&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879239999&partnerID=8YFLogxK

U2 - 10.1016/j.jvsv.2013.04.001

DO - 10.1016/j.jvsv.2013.04.001

M3 - Article

AN - SCOPUS:84879239999

VL - 1

SP - 289

EP - 297

JO - Journal of Vascular Surgery: Venous and Lymphatic Disorders

JF - Journal of Vascular Surgery: Venous and Lymphatic Disorders

SN - 2213-333X

IS - 3

ER -