Effective induction of death in mesothelioma cells with magnetite nanoparticles under an alternating magnetic field

Shofu Matsuda, Eri Nakajima, Takuya Nakanishi, Airi Hitsuji, Hong Zhang, Akane Tanaka, Hiroshi Matsuda, Toshiyuki Momma, Tetsuya Osaka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

With the objective of finding an avenue for development of magnetic hyperthermia as an effective mesothelioma treatment, the influence of heating by magnetite nanoparticles (MNPs) with a diameter of ~ 40 nm, which were incorporated into cells and then subjected to AC magnetic field, on induction of cell death was investigated in all three histological subtypes of human mesothelioma cells (i.e., epithelioid NCI-H28, sarcomatoid NCI-H2052, and biphasic MSTO-211H cells). Cellular uptake of MNPs was observed in all cell types, but the amount of MNPs incorporated per cell into MSTO-211H cells was smaller than in NCI-H28 and NCI-H2052 cells. On the other hand, cell death induced by cellular uptake of MNPs was observed specifically in MSTO-211H cells. Hence, when cells are heated by intracellular MNPs under AC magnetic field, a high degree of cell mortality in NCI-H28 and NCI-H2052 cells is induced by the temperature increase derived from the high amount of intracellular MNPs, but the combination of intracellular heating and cell-type-specific toxicity of MNPs induced high rates of cell death in MSTO-211H cells even at a lower temperature. Almost all of the heated cells were dead after 24-h incubation at 37 °C in all histological subtypes. Additionally, higher mortalities were observed in all three types of mesothelioma cells after MNPs-heating, as compared to the heating with a thermostatic bath. Herein, the significance of cellular uptake of MNPs for effectively inducing cell death in mesothelioma has been demonstrated in vitro.

Original languageEnglish
Pages (from-to)90-96
Number of pages7
JournalMaterials Science and Engineering C
Volume81
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Magnetite Nanoparticles
Magnetite nanoparticles
death
magnetite
induction
Magnetic fields
nanoparticles
cells
magnetic fields
Cell death
Heating
heating
mortality
alternating current
Toxicity

Keywords

  • Alternating magnetic field
  • Cellular uptake
  • Magnetite nanoparticles
  • Mesothelioma

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Effective induction of death in mesothelioma cells with magnetite nanoparticles under an alternating magnetic field. / Matsuda, Shofu; Nakajima, Eri; Nakanishi, Takuya; Hitsuji, Airi; Zhang, Hong; Tanaka, Akane; Matsuda, Hiroshi; Momma, Toshiyuki; Osaka, Tetsuya.

In: Materials Science and Engineering C, Vol. 81, 01.12.2017, p. 90-96.

Research output: Contribution to journalArticle

Matsuda, Shofu ; Nakajima, Eri ; Nakanishi, Takuya ; Hitsuji, Airi ; Zhang, Hong ; Tanaka, Akane ; Matsuda, Hiroshi ; Momma, Toshiyuki ; Osaka, Tetsuya. / Effective induction of death in mesothelioma cells with magnetite nanoparticles under an alternating magnetic field. In: Materials Science and Engineering C. 2017 ; Vol. 81. pp. 90-96.
@article{053e851571e3411292cd6ce0f6d88aca,
title = "Effective induction of death in mesothelioma cells with magnetite nanoparticles under an alternating magnetic field",
abstract = "With the objective of finding an avenue for development of magnetic hyperthermia as an effective mesothelioma treatment, the influence of heating by magnetite nanoparticles (MNPs) with a diameter of ~ 40 nm, which were incorporated into cells and then subjected to AC magnetic field, on induction of cell death was investigated in all three histological subtypes of human mesothelioma cells (i.e., epithelioid NCI-H28, sarcomatoid NCI-H2052, and biphasic MSTO-211H cells). Cellular uptake of MNPs was observed in all cell types, but the amount of MNPs incorporated per cell into MSTO-211H cells was smaller than in NCI-H28 and NCI-H2052 cells. On the other hand, cell death induced by cellular uptake of MNPs was observed specifically in MSTO-211H cells. Hence, when cells are heated by intracellular MNPs under AC magnetic field, a high degree of cell mortality in NCI-H28 and NCI-H2052 cells is induced by the temperature increase derived from the high amount of intracellular MNPs, but the combination of intracellular heating and cell-type-specific toxicity of MNPs induced high rates of cell death in MSTO-211H cells even at a lower temperature. Almost all of the heated cells were dead after 24-h incubation at 37 °C in all histological subtypes. Additionally, higher mortalities were observed in all three types of mesothelioma cells after MNPs-heating, as compared to the heating with a thermostatic bath. Herein, the significance of cellular uptake of MNPs for effectively inducing cell death in mesothelioma has been demonstrated in vitro.",
keywords = "Alternating magnetic field, Cellular uptake, Magnetite nanoparticles, Mesothelioma",
author = "Shofu Matsuda and Eri Nakajima and Takuya Nakanishi and Airi Hitsuji and Hong Zhang and Akane Tanaka and Hiroshi Matsuda and Toshiyuki Momma and Tetsuya Osaka",
year = "2017",
month = "12",
day = "1",
doi = "10.1016/j.msec.2017.07.023",
language = "English",
volume = "81",
pages = "90--96",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Effective induction of death in mesothelioma cells with magnetite nanoparticles under an alternating magnetic field

AU - Matsuda, Shofu

AU - Nakajima, Eri

AU - Nakanishi, Takuya

AU - Hitsuji, Airi

AU - Zhang, Hong

AU - Tanaka, Akane

AU - Matsuda, Hiroshi

AU - Momma, Toshiyuki

AU - Osaka, Tetsuya

PY - 2017/12/1

Y1 - 2017/12/1

N2 - With the objective of finding an avenue for development of magnetic hyperthermia as an effective mesothelioma treatment, the influence of heating by magnetite nanoparticles (MNPs) with a diameter of ~ 40 nm, which were incorporated into cells and then subjected to AC magnetic field, on induction of cell death was investigated in all three histological subtypes of human mesothelioma cells (i.e., epithelioid NCI-H28, sarcomatoid NCI-H2052, and biphasic MSTO-211H cells). Cellular uptake of MNPs was observed in all cell types, but the amount of MNPs incorporated per cell into MSTO-211H cells was smaller than in NCI-H28 and NCI-H2052 cells. On the other hand, cell death induced by cellular uptake of MNPs was observed specifically in MSTO-211H cells. Hence, when cells are heated by intracellular MNPs under AC magnetic field, a high degree of cell mortality in NCI-H28 and NCI-H2052 cells is induced by the temperature increase derived from the high amount of intracellular MNPs, but the combination of intracellular heating and cell-type-specific toxicity of MNPs induced high rates of cell death in MSTO-211H cells even at a lower temperature. Almost all of the heated cells were dead after 24-h incubation at 37 °C in all histological subtypes. Additionally, higher mortalities were observed in all three types of mesothelioma cells after MNPs-heating, as compared to the heating with a thermostatic bath. Herein, the significance of cellular uptake of MNPs for effectively inducing cell death in mesothelioma has been demonstrated in vitro.

AB - With the objective of finding an avenue for development of magnetic hyperthermia as an effective mesothelioma treatment, the influence of heating by magnetite nanoparticles (MNPs) with a diameter of ~ 40 nm, which were incorporated into cells and then subjected to AC magnetic field, on induction of cell death was investigated in all three histological subtypes of human mesothelioma cells (i.e., epithelioid NCI-H28, sarcomatoid NCI-H2052, and biphasic MSTO-211H cells). Cellular uptake of MNPs was observed in all cell types, but the amount of MNPs incorporated per cell into MSTO-211H cells was smaller than in NCI-H28 and NCI-H2052 cells. On the other hand, cell death induced by cellular uptake of MNPs was observed specifically in MSTO-211H cells. Hence, when cells are heated by intracellular MNPs under AC magnetic field, a high degree of cell mortality in NCI-H28 and NCI-H2052 cells is induced by the temperature increase derived from the high amount of intracellular MNPs, but the combination of intracellular heating and cell-type-specific toxicity of MNPs induced high rates of cell death in MSTO-211H cells even at a lower temperature. Almost all of the heated cells were dead after 24-h incubation at 37 °C in all histological subtypes. Additionally, higher mortalities were observed in all three types of mesothelioma cells after MNPs-heating, as compared to the heating with a thermostatic bath. Herein, the significance of cellular uptake of MNPs for effectively inducing cell death in mesothelioma has been demonstrated in vitro.

KW - Alternating magnetic field

KW - Cellular uptake

KW - Magnetite nanoparticles

KW - Mesothelioma

UR - http://www.scopus.com/inward/record.url?scp=85026462212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026462212&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2017.07.023

DO - 10.1016/j.msec.2017.07.023

M3 - Article

VL - 81

SP - 90

EP - 96

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -