Effects of 2-deoxy-d-glucose and quinidine on the fruiting body formation in Coprinus Macrorhizus

Isao Uno, Hiroshi Nyunoya, Tatsuo Ishikawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The addition of 2-deoxy-D-glucose and quinidine to culture media of strain Fisc of Coprinus macrorhizus was effective in inhibiting fruiting body formation and in reducing the intracellular level of cyclic AMP, adenylate cyclase and phosphodiesterase activities. 2-Deoxy-D-glucose taken into mycelial cells was phosphorylated and accumulated as 2-deoxy-D-glucose-monophosphate and 2-deoxy-D-glucose diphosphate. This phosphorylation appeared to be reversible. Quinidine was taken up by mycelial cells without further metabolism. 2-Deoxy-D-glucose, 2-deoxy-D-glucosemono-phosphate and quinidine inhibited adenylate cyclase activity, but not phosphodiesterase activity measured in vitro. The Ki values of 2-deoxy-D-glucose and quinidine for adenylate cyclase activity were 1.8 and 0.08 mM, respectively. The data indicate a possible relationship between the low adenylate cyclase activity and inhibition of fruiting body formation.

Original languageEnglish
Pages (from-to)219-228
Number of pages10
JournalThe Journal of General and Applied Microbiology
Volume27
Issue number3
DOIs
Publication statusPublished - 1981
Externally publishedYes

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Coprinus
Quinidine
Deoxyglucose
Adenylyl Cyclases
Glucose
Phosphoric Diester Hydrolases
Diphosphates
Cyclic AMP
Culture Media
Phosphates
Phosphorylation

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Effects of 2-deoxy-d-glucose and quinidine on the fruiting body formation in Coprinus Macrorhizus. / Uno, Isao; Nyunoya, Hiroshi; Ishikawa, Tatsuo.

In: The Journal of General and Applied Microbiology, Vol. 27, No. 3, 1981, p. 219-228.

Research output: Contribution to journalArticle

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N2 - The addition of 2-deoxy-D-glucose and quinidine to culture media of strain Fisc of Coprinus macrorhizus was effective in inhibiting fruiting body formation and in reducing the intracellular level of cyclic AMP, adenylate cyclase and phosphodiesterase activities. 2-Deoxy-D-glucose taken into mycelial cells was phosphorylated and accumulated as 2-deoxy-D-glucose-monophosphate and 2-deoxy-D-glucose diphosphate. This phosphorylation appeared to be reversible. Quinidine was taken up by mycelial cells without further metabolism. 2-Deoxy-D-glucose, 2-deoxy-D-glucosemono-phosphate and quinidine inhibited adenylate cyclase activity, but not phosphodiesterase activity measured in vitro. The Ki values of 2-deoxy-D-glucose and quinidine for adenylate cyclase activity were 1.8 and 0.08 mM, respectively. The data indicate a possible relationship between the low adenylate cyclase activity and inhibition of fruiting body formation.

AB - The addition of 2-deoxy-D-glucose and quinidine to culture media of strain Fisc of Coprinus macrorhizus was effective in inhibiting fruiting body formation and in reducing the intracellular level of cyclic AMP, adenylate cyclase and phosphodiesterase activities. 2-Deoxy-D-glucose taken into mycelial cells was phosphorylated and accumulated as 2-deoxy-D-glucose-monophosphate and 2-deoxy-D-glucose diphosphate. This phosphorylation appeared to be reversible. Quinidine was taken up by mycelial cells without further metabolism. 2-Deoxy-D-glucose, 2-deoxy-D-glucosemono-phosphate and quinidine inhibited adenylate cyclase activity, but not phosphodiesterase activity measured in vitro. The Ki values of 2-deoxy-D-glucose and quinidine for adenylate cyclase activity were 1.8 and 0.08 mM, respectively. The data indicate a possible relationship between the low adenylate cyclase activity and inhibition of fruiting body formation.

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